AB153700

Anti-SPRED2 antibody

Name: Anti-SPRED2 antibody (ab153700) | Abcam
Brand: Abcam Limited
SKU: AB153700

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(9 Publications )

Rabbit Polyclonal SPRED2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Sprouty-related, EVH1 domain-containing protein 2 aa 50-450.

View Alternative Names

Spred-2, SPRED2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRED2 antibody (AB153700)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRED2 antibody (AB153700)

Immunohistochemical analysis of paraffin-embedded Human Breast carcinoma tissue labeling SPRED2 with ab153700 at 1/500 dilution.

Unknown

Western blot - Anti-SPRED2 antibody (AB153700)

10% SDS PAGE

All lanes:

Western blot - Anti-SPRED2 antibody (ab153700) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Sprouty-related, EVH1 domain-containing protein 2 aa 50-450. The exact immunogen used to generate this antibody is proprietary information.

Q7Z698

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negatively regulates Ras signaling pathways and downstream activation of MAP kinases (PubMed : 15683364, PubMed : 34626534). Recruits and translocates NF1 to the cell membrane, thereby enabling NF1-dependent hydrolysis of active GTP-bound Ras to inactive GDP-bound Ras (PubMed : 34626534). Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2 (By similarity). Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells (By similarity).

See full target information Sprouty-related, EVH1 domain-containing protein 2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 38:1269-1282 PubMed36704943

2023

microRNA-141-3p mediates epithelial cell proliferation, apoptosis, and epithelial-mesenchymal transition and alleviates pulmonary fibrosis in mice via Spred2.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhu,Mo Chen,Wenwen Wang,Jianing Zhu,Huaxiang Wu

Bioengineered 13:13882-13892 PubMed35707829

2022

Sprouty-related proteins with EVH1 domain (SPRED2) prevents high-glucose induced endothelial-mesenchymal transition and endothelial injury by suppressing MAPK activation.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Liu,Jing Zhao,Chengmin Lin

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 31:1011-1022 PubMed35506187

2022

MiR-218 promotes oxidative stress and inflammatory response by inhibiting SPRED2-mediated autophagy in HG-induced HK-2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lanfang Fu,Xinxin Huang,Juyun Zhang,Zhu Lin,Guijun Qin

Experimental and therapeutic medicine 22:1352 PubMed34659498

2021

MicroRNA-218 aggravates HO-induced damage in PC12 cells via spred2-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Duoping Chen,Chunmei Li,Rui Lv

Experimental and therapeutic medicine 22:1107 PubMed34504561

2021

Thrombin aggravates hypoxia/reoxygenation injury of astrocytes by activating the autophagy pathway mediated by SPRED2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoning Wang,Weiwei Lu,Bing Liu,Yunhe Xu

Bioscience, biotechnology, and biochemistry 84:2458-2465 PubMed32841583

2020

LncRNA TUG1 reverses LPS-induced cell apoptosis and inflammation of macrophage via targeting MiR-221-3p/SPRED2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Hu,Hongwei Ye,Jianjun Liao

Experimental eye research 178:160-175 PubMed30290165

2018

Spred negatively regulates lens growth by modulating epithelial cell proliferation and fiber differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa Susanto,Guannan Zhao,Fatima Wazin,Yue Feng,John E J Rasko,Charles G Bailey,Frank J Lovicu

Experimental eye research 170:148-159 PubMed29501879

2018

Negative regulation of lens fiber cell differentiation by RTK antagonists Spry and Spred.

Applications

Species

Unspecified reactive species

Guannan Zhao,Charles G Bailey,Yue Feng,John Rasko,Frank J Lovicu

Cancer discovery 8:59-73 PubMed29054992

2017

Suppression of Adaptive Responses to Targeted Cancer Therapy by Transcriptional Repression.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Rusan,Kapsok Li,Yvonne Li,Camilla L Christensen,Brian J Abraham,Nicholas Kwiatkowski,Kevin A Buczkowski,Bruno Bockorny,Ting Chen,Shuai Li,Kevin Rhee,Haikuo Zhang,Wankun Chen,Hideki Terai,Tiffany Tavares,Alan L Leggett,Tianxia Li,Yichen Wang,Tinghu Zhang,Tae-Jung Kim,Sook-Hee Hong,Neermala Poudel-Neupane,Michael Silkes,Tenny Mudianto,Li Tan,Takeshi Shimamura,Matthew Meyerson,Adam J Bass,Hideo Watanabe,Nathanael S Gray,Richard A Young,Kwok-Kin Wong,Peter S Hammerman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPRED2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRED2 antibody (AB153700)

Western blot - Anti-SPRED2 antibody (AB153700)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise