AB59785

Anti-Sprouty 4/Spry-4 antibody

Name: Anti-Sprouty 4/Spry-4 antibody (ab59785) | Abcam
Brand: Abcam Limited
SKU: AB59785
Price: 525 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal Sprouty 4/Spry-4 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 6 publications.

View Alternative Names

Protein sprouty homolog 4, Spry-4, SPRY4

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts specifically with human 32 kDa Sprouty 4/Spry-4 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Spry-4 protein also known as Sprouty 4 is an inhibitor of receptor tyrosine kinase signaling pathways. It weighs approximately 35 kDa and plays a role in modulating fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling. Spry-4 is expressed in various tissues including the brain lungs and kidneys. Its expression varies during different developmental stages and under different physiological conditions.

Biological function summary

Sprouty family proteins such as Spry-4 negatively regulate cell growth differentiation and angiogenesis. This protein functions as part of a negative feedback loop in growth factor-mediated signaling. By modulating signaling Spry-4 helps maintain balanced cellular responses ensuring cells do not undergo uncontrolled proliferation or differentiation. Spry-4's regulatory role is important in tissue development and repair.

Pathways

Spry-4 plays a significant role in the MAPK/ERK and PI3K/AKT pathways. In these pathways Spry-4 interacts with other proteins like RAF1 and Erk. Its inhibitory effects prevent excessive activation of these pathways which are essential for processes like cell division and survival. Spry-4's ability to modulate these pathways determines how cells respond to external stimuli maintaining cellular homeostasis.

Spry-4's dysregulation has been linked to cancer and congenital disorders. For example altered expression of Spry-4 can lead to tumorigenesis since the balance in growth factor signaling is disrupted. In cancer Spry-4 often interacts with other regulatory proteins such as SOS1 which further modulates signaling pathways important for tumor growth. In congenital disorders mutations in Spry-4 or related pathways affect normal tissue development leading to developmental anomalies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed : 12027893). Probably impairs the formation of GTP-Ras (PubMed : 12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed : 12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed : 15584898).

See full target information SPRY4

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Epigenetics 18:2145068 PubMed36384366

2022

Sprouty4 is epigenetically upregulated in human colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Alexei J Stuckel,Shuai Zeng,Zhen Lyu,Wei Zhang,Xu Zhang,Urszula Dougherty,Reba Mustafi,Tripti Khare,Qiong Zhang,Trupti Joshi,Marc Bissonnette,Sharad Khare

Aging 13:11665-11677 PubMed33879635

2021

suppresses proliferation and induces apoptosis of colorectal cancer cells by repressing oncogene .

Applications

Unspecified application

Species

Unspecified reactive species

Jia Guo,Huadong Zhu,Qiang Li,Jianhua Dong,Wei Xiong,Kun Yu

Oncology reports 38:2717-2726 PubMed29048686

2017

miR-1908 as a novel prognosis marker of glioma via promoting malignant phenotype and modulating SPRY4/RAF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chai,Huijie Fan,Yanyan Li,Lijuan Song,Xiaoming Jin,Jiezhong Yu,Yanhua Li,Cungen Ma,Ran Zhou

Development & reproduction 20:171-7 PubMed27660833

2016

Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells.

Applications

Species

Unspecified reactive species

Jae-Young Lee,Sunghyun Park,Kwang-Soo Kim,Jeong-Jae Ko,Soohong Lee,Keun Pil Kim,Kyung-Soon Park

The Journal of clinical investigation 126:349-64 PubMed26657862

2015

MicroRNA-31 initiates lung tumorigenesis and promotes mutant KRAS-driven lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mick D Edmonds,Kelli L Boyd,Tamara Moyo,Ramkrishna Mitra,Robert Duszynski,Maria Pia Arrate,Xi Chen,Zhongming Zhao,Timothy S Blackwell,Thomas Andl,Christine M Eischen

Oncogene 29:4800-13 PubMed20543868

2010

SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity.

Applications

Species

Human

A Barbáchano,P Ordóñez-Morán,J M García,A Sánchez,F Pereira,M J Larriba,N Martínez,J Hernández,S Landolfi,F Bonilla,H G Pálmer,J M Rojas,A Muñoz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sprouty 4/Spry-4 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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