AB7513

Anti-Sprouty 4/Spry-4 antibody

Name: Anti-Sprouty 4/Spry-4 antibody (ab7513) | Abcam
Brand: Abcam Limited
SKU: AB7513
Price: 535 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal Sprouty 4/Spry-4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SPRY4 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Protein sprouty homolog 4, Spry-4, SPRY4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

ab7513 staining tumour cells in human liver tissue. Brown color indicates presence of protein, blue color shows cell nuclei.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

ab7513 at 2.5 μg/ml staining human heart tissue.

Unknown

Western blot - Anti-Sprouty 4/Spry-4 antibody (AB7513)

The blot was incubated with ab7516 (1/100) overnight at 4°C.

All lanes:

Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513) at 1/100 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

SW13 whole cell lysate at 30 µg

Lane 3:

C2C12 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution

Predicted band size: 33 kDa

true

Unknown

Western blot - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Western blot using ab7513 at 1/500.

Lane 1 : Hela nuclear
Lane 2 : Hela
Lane 3 : A431
Lane 4 : Jurkat
Lane 5 : 293

Secondary ab : Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time : 2minutes.
Lysates at 5μg per lane.

We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.

Western blot using ab7513 at 1/500.

Lane 1 : Hela nuclear
Lane 2 : Hela
Lane 3 : A431
Lane 4 : Jurkat
Lane 5 : 293

Secondary ab : Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time : 2minutes.
Lysates at 5μg per lane.

We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.

All lanes:

Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513)

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SPRY4 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9C004

Specificity

A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-10 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This affinity purified antibody is directed against human Sprouty-4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Spry-4 protein also known as Sprouty 4 is an inhibitor of receptor tyrosine kinase signaling pathways. It weighs approximately 35 kDa and plays a role in modulating fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling. Spry-4 is expressed in various tissues including the brain lungs and kidneys. Its expression varies during different developmental stages and under different physiological conditions.

Biological function summary

Sprouty family proteins such as Spry-4 negatively regulate cell growth differentiation and angiogenesis. This protein functions as part of a negative feedback loop in growth factor-mediated signaling. By modulating signaling Spry-4 helps maintain balanced cellular responses ensuring cells do not undergo uncontrolled proliferation or differentiation. Spry-4's regulatory role is important in tissue development and repair.

Pathways

Spry-4 plays a significant role in the MAPK/ERK and PI3K/AKT pathways. In these pathways Spry-4 interacts with other proteins like RAF1 and Erk. Its inhibitory effects prevent excessive activation of these pathways which are essential for processes like cell division and survival. Spry-4's ability to modulate these pathways determines how cells respond to external stimuli maintaining cellular homeostasis.

Spry-4's dysregulation has been linked to cancer and congenital disorders. For example altered expression of Spry-4 can lead to tumorigenesis since the balance in growth factor signaling is disrupted. In cancer Spry-4 often interacts with other regulatory proteins such as SOS1 which further modulates signaling pathways important for tumor growth. In congenital disorders mutations in Spry-4 or related pathways affect normal tissue development leading to developmental anomalies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed : 12027893). Probably impairs the formation of GTP-Ras (PubMed : 12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed : 12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed : 15584898).

See full target information SPRY4

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 58:102513 PubMed36334381

2022

Macrophage Sprouty4 deficiency diminishes sepsis-induced acute lung injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Chen,Chen Cao,Huimin Liu,Wanli Jiang,Rui Pan,He He,Ke Ding,Qingtao Meng

Tumour biology : the journal of the International 37:9197-207 PubMed26768617

2016

Sprouty4 mediates amphiregulin-induced down-regulation of E-cadherin and cell invasion in human ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wai-Kin So,Jung-Chien Cheng,Yingtao Liu,Congjian Xu,Jianfang Zhao,Vincent T W Chang,Peter C K Leung

Oncogene 29:4800-13 PubMed20543868

2010

SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity.

Applications

Species

Human

A Barbáchano,P Ordóñez-Morán,J M García,A Sánchez,F Pereira,M J Larriba,N Martínez,J Hernández,S Landolfi,F Bonilla,H G Pálmer,J M Rojas,A Muñoz

Physiological genomics 28:193-202 PubMed16985006

2006

Microarray interrogation of human metanephric mesenchymal cells highlights potentially important molecules in vivo.

Applications

IHC-P

Species

Human

Karen L Price,David A Long,Nipurna Jina,Helen Liapis,Mike Hubank,Adrian S Woolf,Paul J D Winyard

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sprouty 4/Spry-4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Western blot - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Western blot - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise