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Rabbit Recombinant Monoclonal Sprouty 4/Spry-4 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (AB176337), expandable thumbnail
  • Western blot - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (AB176337), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Not recommended
Mouse
Predicted
Not recommended
Expected
Not recommended
Not recommended
Rat
Predicted
Not recommended
Expected
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

-

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed:12027893). Probably impairs the formation of GTP-Ras (PubMed:12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed:12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:15584898).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Sprouty 4/Spry-4 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR12127

Purity

Tissue culture supernatant

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Spry-4 protein also known as Sprouty 4 is an inhibitor of receptor tyrosine kinase signaling pathways. It weighs approximately 35 kDa and plays a role in modulating fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling. Spry-4 is expressed in various tissues including the brain lungs and kidneys. Its expression varies during different developmental stages and under different physiological conditions.

Biological function summary

Sprouty family proteins such as Spry-4 negatively regulate cell growth differentiation and angiogenesis. This protein functions as part of a negative feedback loop in growth factor-mediated signaling. By modulating signaling Spry-4 helps maintain balanced cellular responses ensuring cells do not undergo uncontrolled proliferation or differentiation. Spry-4's regulatory role is important in tissue development and repair.

Pathways

Spry-4 plays a significant role in the MAPK/ERK and PI3K/AKT pathways. In these pathways Spry-4 interacts with other proteins like RAF1 and Erk. Its inhibitory effects prevent excessive activation of these pathways which are essential for processes like cell division and survival. Spry-4's ability to modulate these pathways determines how cells respond to external stimuli maintaining cellular homeostasis.

Associated diseases and disorders

Spry-4's dysregulation has been linked to cancer and congenital disorders. For example altered expression of Spry-4 can lead to tumorigenesis since the balance in growth factor signaling is disrupted. In cancer Spry-4 often interacts with other regulatory proteins such as SOS1 which further modulates signaling pathways important for tumor growth. In congenital disorders mutations in Spry-4 or related pathways affect normal tissue development leading to developmental anomalies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunoprecipitation - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337), expandable thumbnail

    Immunoprecipitation - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)

    Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)

    Predicted band size: 33 kDa

  • Western blot - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337), expandable thumbnail

    Western blot - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)

    All lanes: Western blot - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: MCF7 cell lysate at 10 µg

    Lane 3: HeLa cell lysate at 10 µg

    Lane 4: K562 cell lysate at 10 µg

    Predicted band size: 33 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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