Anti-Spry-2 antibody [EPR4318(2)(B)]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] (AB180527)

Immunofluorescence analysis of paraformaldehyde-fixed SH-SY5Y cells labeling Spry-2 with ab180527 at 1/1000 dilution. Goat anti-rabbit IgG (Dylight 488) at 1/250 dilution was used as the secondary antibody. The slide is counterstained with Dapi.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] (AB180527)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Spry-2 with ab180527 at 1/50 dilution. The retrieval buffer was 0.01 M Tris-EDTA (pH 9.0) and HIER was performed in a Decloaker (125°C 30 sec cool down to room temperature for 10-30 min). The slide is counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (AB180527)

All lanes:

Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

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• WB

Supplier Data

Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (AB180527)

Western blot analysis of Hela cell lysate immunoprecipitated with ab180527. Detection utilised ab180527 at 1/40 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. The blocking buffer and dilution buffer was 5% NFDM/TBST.

All lanes:

Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527)

Predicted band size: 35 kDa

false

• WB

Supplier Data

Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (AB180527)

All lanes:

Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/2000 dilution

Lane 1:

HT-29 cell lysate at 20 µg

Lane 2:

PC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

false

• WB

Supplier Data

Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (AB180527)

Blocking and dilution buffer : 5% NFDM/TBST.

Low expression control : rat spleen (reference HPA protein expression data).

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat testis tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Exposure time: 180s