Rabbit Recombinant Monoclonal Spry-2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat samples.
Images
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--western-blot-img69307.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224)")
![Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--immunocytochemistry-immunofluorescence-img206.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100403.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224)")
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--western-blot-img129812.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224)")
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--western-blot-img61461.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (AB250224)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Expected | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2 (By similarity). Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis (By similarity). Inhibits TGFB-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells (By similarity). Inhibits CBL/C-CBL-mediated EGFR ubiquitination (PubMed:17974561).
Alternative names
Protein sprouty homolog 2, Spry-2, SPRY2
Recommended products
Rabbit Recombinant Monoclonal Spry-2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR4318(2)(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250224 is the carrier-free version of Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Spry-2 also known as Sprouty homolog 2 is an intracellular protein involved in the regulation of signaling pathways. It has a molecular weight of approximately 35 kDa. This protein expresses in tissues such as the lung kidney and colon. Mainly Spry-2 acts through interaction with other proteins to inhibit or modulate various cellular processes such as growth and differentiation.
- Biological function summary
Sprouty homolog 2 functions by mediating negative feedback in signaling pathways. It plays a role in controlling cellular responses to growth factors. Although not part of a large protein complex it interacts with specific proteins to exert its biological effects. For instance Spry-2 interacts with components of the receptor tyrosine kinase signaling pathway. This interaction helps fine-tune cell signaling responses preventing excessive cell proliferation.
- Pathways
Sprouty homolog 2 acts within the MAPK/ERK signaling pathway and the fibroblast growth factor (FGF) pathway. In these pathways Spry-2 functions as an inhibitor thereby maintaining proper cellular regulation. It interacts with proteins like Raf and inhibits their activities ensuring balanced signal transduction. In the MAPK/ERK pathway this regulation by Spry-2 helps control cellular responses to external stimuli.
- Associated diseases and disorders
Abnormalities in Sprouty homolog 2 function relate to cancer and congenital anomalies. Altered expression or mutations in Spry-2 have associations with colorectal cancer partly due to its role in cell signaling regulation. Spry-2 connects to proteins like Ras known for their involvement in oncogenic pathways. Similarly variations in Spry-2 activity can lead to developmental disorders highlighting its importance in normal cellular function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--western-blot-img69307.jpg "Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)")
Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
This data was developed using Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527, the same antibody clone in a different buffer formulation.
Western blot analysis of HeLa cell lysate immunoprecipitated with Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527. Detection utilised Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527 at 1/40 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527)
Predicted band size: 35 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--immunocytochemistry-immunofluorescence-img206.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)")
Immunocytochemistry/ Immunofluorescence - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
This data was developed using Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of paraformaldehyde-fixed SH-SY5Y cells labeling Spry-2 with Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527 at 1/1000 dilution. Goat anti-rabbit IgG (Dylight 488) at 1/250 dilution was used as the secondary antibody. The slide is counterstained with Dapi.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100403.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
This data was developed using Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Spry-2 with Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527 at 1/50 dilution. The retrieval buffer was 0.01 M Tris-EDTA (pH 9.0) and HIER was performed in a Decloaker (125°C, 30 sec, cool down to room temperature for 10-30 min). The slide is counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--western-blot-img129812.jpg "Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)")
Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
This data was developed using Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527) at 1/10000 dilution
Lane 1: HeLa cell lysate at 20 µg
Lane 2: SH-SY5Y cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--western-blot-img61461.jpg "Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)")
Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
This data was developed using Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527) at 1/2000 dilution
Lane 1: HT-29 cell lysate at 20 µg
Lane 2: PC-3 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDa
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--western-blot-img430428.jpg "Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)")
Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
This data was developed using Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Low expression control: rat spleen (reference HPA protein expression data).
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
All lanes: Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] (Anti-Spry-2 antibody [EPR4318(2)(B)] ab180527) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat liver tissue lysate at 20 µg
Lane 3: Rat testis tissue lysate at 20 µg
Lane 4: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 180s
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224), expandable thumbnail](https://content.abcam.com/products/images/spry-2-antibody-epr43182b-bsa-and-azide-free-ab250224--western-blot-img430428.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)")
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