Rabbit Polyclonal SPRY3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Protein sprouty homolog 3 aa 50 to C-terminus.
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Publications
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- EBioMedicine 50:166-1772019Sprouty4 correlates with favorable prognosis in perihilar cholangiocarcinoma by blocking the FGFR-ERK signaling pathway and arresting the cell cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Qiu et. al.PubMed 31761616
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Protein sprouty homolog 3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43610
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-2 µg/mL | Notes - |
Species Rat | Dilution info 0.5-2 µg/mL | Notes - |
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Inhibits neurite branching, arbor length and neurite complexity (By similarity). Inhibits EGF-mediated p42/44 ERK signaling (By similarity). Negatively regulates the MAPK cascade, resulting in a reduction of extracellular matrix protein accumulation (PubMed:30878395). May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis (PubMed:9458049).
Alternative names
Protein sprouty homolog 3, Spry-3, Sprouty RTK signaling antagonist 3, Sprouty3, SPRY3
Recommended products
Rabbit Polyclonal SPRY3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Protein sprouty homolog 3 aa 50 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Protein sprouty homolog 3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43610
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Western blot - Anti-SPRY3 antibody (ab233424)
All lanes: Western blot - Anti-SPRY3 antibody (ab233424) at 2 µg/mL
All lanes: Rat cerebrum lysate
SecondaryAll lanes: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL
Predicted band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (ab233424)
Formalin-fixed, paraffin embedded human liver tissue stained for SPRY3 using ab233424 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (ab233424)
Formalin-fixed, paraffin embedded human liver cancer tissue stained for SPRY3 using ab233424 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (ab233424)
Formalin-fixed, paraffin embedded human renal cancer tissue stained for SPRY3 using ab233424 at 20 μg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.
Western blot - Anti-SPRY3 antibody (ab233424)
All lanes: Western blot - Anti-SPRY3 antibody (ab233424) at 2 µg/mL
All lanes: Mouse cerebrum lysate
SecondaryAll lanes: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL
Predicted band size: 31 kDa
Western blot - Anti-SPRY3 antibody (ab233424)
All lanes: Western blot - Anti-SPRY3 antibody (ab233424) at 3 µg/mL
All lanes: Human lung lysate
SecondaryAll lanes: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL
Predicted band size: 31 kDa
Western blot - Anti-SPRY3 antibody (ab233424)
All lanes: Western blot - Anti-SPRY3 antibody (ab233424) at 2 µg/mL
All lanes: Recombinant human SPRY3 protein
Predicted band size: 31 kDa
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