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AB233424

Anti-SPRY3 antibody

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(2 Publications)

Rabbit Polyclonal SPRY3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Protein sprouty homolog 3 aa 50 to C-terminus.

View Alternative Names

Protein sprouty homolog 3, Spry-3, Sprouty RTK signaling antagonist 3, Sprouty3, SPRY3

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (AB233424)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (AB233424)

Formalin-fixed, paraffin embedded human liver tissue stained for SPRY3 using ab233424 at 20 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (AB233424)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (AB233424)

Formalin-fixed, paraffin embedded human liver cancer tissue stained for SPRY3 using ab233424 at 20 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (AB233424)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPRY3 antibody (AB233424)

Formalin-fixed, paraffin embedded human renal cancer tissue stained for SPRY3 using ab233424 at 20 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Western blot - Anti-SPRY3 antibody (AB233424)
  • WB

Supplier Data

Western blot - Anti-SPRY3 antibody (AB233424)

All lanes:

Western blot - Anti-SPRY3 antibody (ab233424) at 3 µg/mL

All lanes:

Human lung lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 31 kDa

false

Western blot - Anti-SPRY3 antibody (AB233424)
  • WB

Supplier Data

Western blot - Anti-SPRY3 antibody (AB233424)

All lanes:

Western blot - Anti-SPRY3 antibody (ab233424) at 2 µg/mL

All lanes:

Rat cerebrum lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 31 kDa

false

Western blot - Anti-SPRY3 antibody (AB233424)
  • WB

Supplier Data

Western blot - Anti-SPRY3 antibody (AB233424)

All lanes:

Western blot - Anti-SPRY3 antibody (ab233424) at 2 µg/mL

All lanes:

Recombinant human SPRY3 protein

Predicted band size: 31 kDa

false

Western blot - Anti-SPRY3 antibody (AB233424)
  • WB

Supplier Data

Western blot - Anti-SPRY3 antibody (AB233424)

All lanes:

Western blot - Anti-SPRY3 antibody (ab233424) at 2 µg/mL

All lanes:

Mouse cerebrum lysate

Secondary

All lanes:

HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Protein sprouty homolog 3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43610

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits neurite branching, arbor length and neurite complexity (By similarity). Inhibits EGF-mediated p42/44 ERK signaling (By similarity). Negatively regulates the MAPK cascade, resulting in a reduction of extracellular matrix protein accumulation (PubMed : 30878395). May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis (PubMed : 9458049).
See full target information Protein sprouty homolog 3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 62:10 PubMed33970198

2021

Role of IL-36γ/IL-36R Signaling in Corneal Innate Defense Against Candida albicans Keratitis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Dai,Rao Me,Nan Gao,Guanyu Su,Xinyi Wu,Fu-Shin X Yu

EBioMedicine 50:166-177 PubMed31761616

2019

Sprouty4 correlates with favorable prognosis in perihilar cholangiocarcinoma by blocking the FGFR-ERK signaling pathway and arresting the cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Qiu,Tianli Chen,Rongqi Sun,Zengli Liu,Xiaoming Zhang,Zhipeng Li,Yunfei Xu,Zongli Zhang
View all publications
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Product promise

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