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AB244429

Anti-SPT5 antibody

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(1 Publication)

Rabbit Polyclonal SPT5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SUPT5H aa 900 to C-terminus.

View Alternative Names

SPT5, SPT5H, SUPT5H, Transcription elongation factor SPT5, hSPT5, DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, Tat-cotransactivator 1 protein, DSIF p160, DSIF large subunit, Tat-CT1 protein

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPT5 antibody (AB244429)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPT5 antibody (AB244429)

Paraffin-embedded human testis tissue stained for SPT5 using ab244429 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPT5 antibody (AB244429)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPT5 antibody (AB244429)

Paraffin-embedded human pancreas tissue stained for SPT5 using ab244429 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SPT5 antibody (AB244429)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SPT5 antibody (AB244429)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SPT5 (green) using ab244429 at 4 μg/ml in ICC/IF.

Western blot - Anti-SPT5 antibody (AB244429)
  • WB

Unknown

Western blot - Anti-SPT5 antibody (AB244429)

All lanes:

Western blot - Anti-SPT5 antibody (ab244429) at 0.4 µg/mL

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 121 kDa

false

Western blot - Anti-SPT5 antibody (AB244429)
  • WB

Unknown

Western blot - Anti-SPT5 antibody (AB244429)

All lanes:

Western blot - Anti-SPT5 antibody (ab244429) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 121 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SUPT5H aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00267

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II (PubMed : 10075709, PubMed : 10199401, PubMed : 10421630, PubMed : 10757782, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A (PubMed : 10075709, PubMed : 10421630, PubMed : 10757782, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter (PubMed : 10075709, PubMed : 10199401, PubMed : 10757782, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex (PubMed : 10075709, PubMed : 10199401, PubMed : 10421630, PubMed : 10757782, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II (PubMed : 16214896). TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme (PubMed : 16214896). Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (PubMed : 16214896). Following phosphorylation by CDK9, DSIF can also positively regulate transcriptional elongation (PubMed : 16427012). Required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat (PubMed : 10393184, PubMed : 10454543, PubMed : 11809800, PubMed : 9514752). DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences (PubMed : 11112772, PubMed : 14701750).
See full target information SUPT5H
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:200-217 PubMed38164169

2024

Treatment for type 2 diabetes and diabetic nephropathy by targeting Smad3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Huijun He,Honglian Wang,Xiaocui Chen,Yu Zhong,Xiao Ru Huang,Ronald Cw Ma,Cheng Wang,Hui-Yao Lan
View all publications
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Product promise

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