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AB245650

Anti-SPT5 antibody

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Rabbit Polyclonal SPT5 antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SUPT5H aa 50-100.

View Alternative Names

SPT5, SPT5H, SUPT5H, Transcription elongation factor SPT5, hSPT5, DRB sensitivity-inducing factor 160 kDa subunit, DRB sensitivity-inducing factor large subunit, Tat-cotransactivator 1 protein, DSIF p160, DSIF large subunit, Tat-CT1 protein

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Immunoprecipitation - Anti-SPT5 antibody (AB245650)
  • IP

Supplier Data

Immunoprecipitation - Anti-SPT5 antibody (AB245650)

SPT5 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab245650 used for IP at 3 μg/mg lysate. For WB another anti-SPT5 antibody was used at 1 μg/ml.

Lane 1 : ab245650 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-SPT5 antibody (ab245650)

Predicted band size: 121 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human SUPT5H aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O00267

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245650 was affinity purified using an epitope specific to SPT5 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II (PubMed : 10075709, PubMed : 10199401, PubMed : 10421630, PubMed : 10757782, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A (PubMed : 10075709, PubMed : 10421630, PubMed : 10757782, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter (PubMed : 10075709, PubMed : 10199401, PubMed : 10757782, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex (PubMed : 10075709, PubMed : 10199401, PubMed : 10421630, PubMed : 10757782, PubMed : 10912001, PubMed : 11112772, PubMed : 11553615, PubMed : 12653964, PubMed : 12718890, PubMed : 15136722, PubMed : 15380072, PubMed : 9450929, PubMed : 9857195). DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II (PubMed : 16214896). TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme (PubMed : 16214896). Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites (PubMed : 16214896). Following phosphorylation by CDK9, DSIF can also positively regulate transcriptional elongation (PubMed : 16427012). Required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat (PubMed : 10393184, PubMed : 10454543, PubMed : 11809800, PubMed : 9514752). DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences (PubMed : 11112772, PubMed : 14701750).
See full target information SUPT5H
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