Rabbit Polyclonal SPTBN1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human SPTBN1.
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- PloS one 18:e02878292023Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, autophagy, and YB-1 independent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomozumi Imamichi,Qian Chen,Bharatwaj Sowrirajan,Jun Yang,Sylvain Laverdure,Mayra Marquez,Anthony R Mele,Catherine Watkins,Joseph W Adelsberger,Jeanette Higgins,Hongyan SuiPubMed 37910521
- Cell genomics 3:1002502023Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGreta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper LagePubMed 36950384
- Frontiers in molecular neuroscience 15:10174042022Analyzing the mechanisms that facilitate the subtype-specific assembly of -aminobutyric acid type A receptors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCatherine Choi,Joshua L Smalley,Abigail H S Lemons,Qiu Ren,Christopher E Bope,Jake S Dengler,Paul A Davies,Stephen J MossPubMed 36263376
- Frontiers in cardiovascular medicine 8:7648312021miR-153-3p Targets βII Spectrin to Regulate Formaldehyde-Induced Cardiomyocyte Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanyu Yang,Yanyan Yang,Xiangqin He,Pin Sun,Ying Zhang,Xiaoxia Song,Yu Tian,Tingyu Zong,Jianmin Ma,Xiaofei Chen,Qifeng Lv,Tao Yu,Zhirong JiangPubMed 34977182
- Journal of cellular and molecular medicine 25:11300-113092021β2SP/TET2 complex regulates gene 5hmC modification after cerebral ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohua Ma,Meng Zhang,Rui Yan,Hainan Wu,Bo Yang,Zhigang MiaoPubMed 34799994
- Frontiers in cell and developmental biology 9:6537242021SPTBN1 Prevents Primary Osteoporosis by Modulating Osteoblasts Proliferation and Differentiation and Blood Vessels Formation in Bone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuejuan Xu,Jiayi Yang,Yanshi Ye,Guoqiang Chen,Yinhua Zhang,Hangtian Wu,Yuqian Song,Meichen Feng,Xiaoting Feng,Xingying Chen,Xiao Wang,Xu Lin,Xiaochun Bai,Jie ShenPubMed 33816505
- Frontiers in molecular neuroscience 13:5630912020Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoshua L Smalley et. al.PubMed 33192291
- Oncotarget 7:38440-384502016Validation of a multi-omics strategy for prioritizing personalized candidate driver genesApplications:Unspecified applicationReactive species:Unspecified reactive speciesLi Liang,Liting Song,Yi Yang,Ling Tian,Xiaoyuan Li,Songfeng Wu,Wenxun Huang,Hong Ren,Ni Tang,Keyue DingPubMed 27469031
- BMC cancer 15:1162015The prognostic value of combined TGF-β1 and ELF in hepatocellular carcinoma.
- Annals of surgical oncology 21 Suppl 4:S505-142014CEACAM1 long cytoplasmic domain isoform is associated with invasion and recurrence of hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShigehisa Kiriyama et. al.PubMed 24390710
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SPTBN1. The exact immunogen used to generate this antibody is proprietary information. Database link Q01082
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Elephant | Predicted | Predicted | Predicted |
| Ferret | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Opossum | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant, Cow, Opossum | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant, Cow, Opossum | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant, Cow, Opossum | Dilution info - | Notes - |
Associated Products
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Target data
Function
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Plays a critical role in central nervous system development and function.
Alternative names
SPTB2, SPTBN1, Beta-II spectrin, Fodrin beta chain
Recommended products
Rabbit Polyclonal SPTBN1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human SPTBN1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SPTBN1. The exact immunogen used to generate this antibody is proprietary information. Database link Q01082
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody (ab72239)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SPTBN1 with ab72239 at 1/1000 (0.2µg/ml). Detection: DAB.
Immunoprecipitation - Anti-SPTBN1 antibody (ab72239)
Immunoprecipitation/ Western Blot of SPTBN1.
Lane 1: ab72239 at 3μg/mg whole cell lysate.
Lane 2: Control IgG.
ab72239 at 1μg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 1 second.All lanes: Immunoprecipitation - Anti-SPTBN1 antibody (ab72239)
Predicted band size: 275 kDa
Western blot - Anti-SPTBN1 antibody (ab72239)
All lanes: Western blot - Anti-SPTBN1 antibody (ab72239) at 0.04 µg/mL
Lane 1: Whole cell lysate from Hela cells at 50 µg
Lane 2: Whole cell lysate from Hela cells at 15 µg
Lane 3: Whole cell lysate from Hela cells at 5 µg
Lane 4: Whole cell lysate from 293T cells at 50 µg
Predicted band size: 275 kDa
Observed band size: 274 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody (ab72239)
IHC image of ab72239 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72239, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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