Rabbit Recombinant Monoclonal SPTBN1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (AB124888), expandable thumbnail](https://content.abcam.com/products/images/sptbn1-antibody-epr5869-ab124888--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130611.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (AB124888)")
![Western blot - Anti-SPTBN1 antibody [EPR5869] (AB124888), expandable thumbnail](https://content.abcam.com/products/images/sptbn1-antibody-epr5869-ab124888--western-blot-img116256.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SPTBN1 antibody [EPR5869] (AB124888)")
![OI-RD Scanning - Anti-SPTBN1 antibody [EPR5869] (AB124888), expandable thumbnail](https://content.abcam.com/products/images/sptbn1-antibody-epr5869-ab124888--oi-rd-scanning-img156649.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-SPTBN1 antibody [EPR5869] (AB124888)")
Publications
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- EBioMedicine 57:1028782020Scleral HIF-1α is a prominent regulatory candidate for genetic and environmental interactions in human myopia pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Zhao et. al.PubMed 32652319
- Brain : a journal of neurology 140:2322-23362017Delineating SPTAN1 associated phenotypes: from isolated epilepsy to encephalopathy with progressive brain atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteffen Syrbe,Frederike L Harms,Elena Parrini,Martino Montomoli,Ulrike Mütze,Katherine L Helbig,Tilman Polster,Beate Albrecht,Ulrich Bernbeck,Ellen van Binsbergen,Saskia Biskup,Lydie Burglen,Jonas Denecke,Bénédicte Heron,Henrike O Heyne,Georg F Hoffmann,Frauke Hornemann,Takeshi Matsushige,Ryuki Matsuura,Mitsuhiro Kato,G Christoph Korenke,Alma Kuechler,Constanze Lämmer,Andreas Merkenschlager,Cyril Mignot,Susanne Ruf,Mitsuko Nakashima,Hirotomo Saitsu,Hannah Stamberger,Tiziana Pisano,Jun Tohyama,Sarah Weckhuysen,Wendy Werckx,Julia Wickert,Francesco Mari,Nienke E Verbeek,Rikke S Møller,Bobby Koeleman,Naomichi Matsumoto,William B Dobyns,Domenica Battaglia,Johannes R Lemke,Kerstin Kutsche,Renzo GuerriniPubMed 29050398
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Expected |
| Rat | Predicted | Not recommended | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes (Heat to 98°C, allow to cool for 10-20 minutes) Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Plays a critical role in central nervous system development and function.
Alternative names
SPTB2, SPTBN1, Beta-II spectrin, Fodrin beta chain
Recommended products
Rabbit Recombinant Monoclonal SPTBN1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5869
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 4.4 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (ab124888), expandable thumbnail](https://content.abcam.com/products/images/sptbn1-antibody-epr5869-ab124888--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130611.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (ab124888)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (ab124888)
ab124888, at 1/250 dilution, staining SPTBN1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-SPTBN1 antibody [EPR5869] (ab124888), expandable thumbnail](https://content.abcam.com/products/images/sptbn1-antibody-epr5869-ab124888--western-blot-img116256.jpg "Western blot - Anti-SPTBN1 antibody [EPR5869] (ab124888)")
Western blot - Anti-SPTBN1 antibody [EPR5869] (ab124888)
All lanes: Western blot - Anti-SPTBN1 antibody [EPR5869] (ab124888) at 1/1000 dilution
Lane 1: SH-SY5Y cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: Caco 2 cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
Predicted band size: 275 kDa
![OI-RD Scanning - Anti-SPTBN1 antibody [EPR5869] (ab124888), expandable thumbnail](https://content.abcam.com/products/images/sptbn1-antibody-epr5869-ab124888--oi-rd-scanning-img156649.jpg "OI-RD Scanning - Anti-SPTBN1 antibody [EPR5869] (ab124888)")
OI-RD Scanning - Anti-SPTBN1 antibody [EPR5869] (ab124888)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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