AB124888

Anti-SPTBN1 antibody [EPR5869]

RabMAb
Recombinant
KO Validated
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal SPTBN1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

SPTB2, SPTBN1, Beta-II spectrin, Fodrin beta chain

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (AB124888)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (AB124888)

ab124888 at 1/250 dilution staining SPTBN1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SPTBN1 antibody [EPR5869] (AB124888)

All lanes:

Western blot - Anti-SPTBN1 antibody [EPR5869] (ab124888) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Caco 2 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 275 kDa

false

OI-RD Scanning - Anti-SPTBN1 antibody [EPR5869] (AB124888)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SPTBN1 antibody [EPR5869] (AB124888)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-SPTBN1 antibody [EPR5869] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5869

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Plays a critical role in central nervous system development and function.

See full target information SPTBN1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 10:e724 PubMed36444616

2022

SPTBN1 attenuates rheumatoid arthritis synovial cell proliferation, invasion, migration and inflammatory response by binding to PIK3R2.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ping Dai,Xiao-Dong Xu,Ting-Ting Yang,Zhi-Hua Yin,Zhi-Zhong Ye,Ya-Zhi Wei

EBioMedicine 57:102878 PubMed32652319

2020

Scleral HIF-1α is a prominent regulatory candidate for genetic and environmental interactions in human myopia pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Zhao,Dake Zhang,Qingyi Zhou,Fuxin Zhao,Mingguang He,Zhenglin Yang,Yongchao Su,Ying Zhai,Jiaofeng Yan,Guoyun Zhang,Anquan Xue,Jing Tang,Xiaotong Han,Yi Shi,Yun Zhu,Tianzi Liu,Wenjuan Zhuang,Lulin Huang,Yaqiang Hong,Deng Wu,Yingxiang Li,Qinkang Lu,Wei Chen,Shiming Jiao,Qiongsi Wang,Nethrajeith Srinivasalu,Yingying Wen,Changqing Zeng,Jia Qu,Xiangtian Zhou

Brain : a journal of neurology 140:2322-2336 PubMed29050398

2017

Delineating SPTAN1 associated phenotypes: from isolated epilepsy to encephalopathy with progressive brain atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Steffen Syrbe,Frederike L Harms,Elena Parrini,Martino Montomoli,Ulrike Mütze,Katherine L Helbig,Tilman Polster,Beate Albrecht,Ulrich Bernbeck,Ellen van Binsbergen,Saskia Biskup,Lydie Burglen,Jonas Denecke,Bénédicte Heron,Henrike O Heyne,Georg F Hoffmann,Frauke Hornemann,Takeshi Matsushige,Ryuki Matsuura,Mitsuhiro Kato,G Christoph Korenke,Alma Kuechler,Constanze Lämmer,Andreas Merkenschlager,Cyril Mignot,Susanne Ruf,Mitsuko Nakashima,Hirotomo Saitsu,Hannah Stamberger,Tiziana Pisano,Jun Tohyama,Sarah Weckhuysen,Wendy Werckx,Julia Wickert,Francesco Mari,Nienke E Verbeek,Rikke S Møller,Bobby Koeleman,Naomichi Matsumoto,William B Dobyns,Domenica Battaglia,Johannes R Lemke,Kerstin Kutsche,Renzo Guerrini

PloS one 10:e0126562 PubMed25978455

2015

Transcriptional Dynamics of Immortalized Human Mesenchymal Stem Cells during Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Masao Takeuchi,Atsunori Higashino,Kikuko Takeuchi,Yutaro Hori,Kazuko Koshiba-Takeuchi,Hatsune Makino,Yoko Monobe,Marina Kishida,Jun Adachi,Jun Takeuchi,Takeshi Tomonaga,Akihiro Umezawa,Yosuke Kameoka,Ken-Ichi Akagi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPTBN1 antibody [EPR5869]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (AB124888)

Western blot - Anti-SPTBN1 antibody [EPR5869] (AB124888)

OI-RD Scanning - Anti-SPTBN1 antibody [EPR5869] (AB124888)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

Product promise