Anti-SPTBN2 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-SPTBN2 antibody (AB264178)

SPTBN2 was immunoprecipitated from 1mg of HeLa cells (20% of IP loaded) using ab264178 at 6 μg per reaction. Western blot was performed on the immunprecipitate using ab264178 at 0.4 μg/ml.

Lane 1 : ab264178 in HeLa whole cell lysate

Lane 2 : Control IgG in HeLa whole cell lysate

Detected with chemilimuniscence with exposure time of 3 minutes.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-SPTBN2 antibody (ab264178)

Predicted band size: 271 kDa

false

• WB

Supplier Data

Western blot - Anti-SPTBN2 antibody (AB264178)

4 - 8% SDS-PAGE.

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-SPTBN2 antibody (ab264178) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T whole cell lysate at 50 µg

Lane 4:

HEK-293T whole cell lysate at 15 µg

Secondary

All lanes:

Goat anti-Rabbit Light Chain HRP Conjugate

Predicted band size: 271 kDa

true

Exposure time: 3min