Rabbit Polyclonal SPTBN2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SPTBN2 aa 2300 to C-terminus.
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- Experimental and therapeutic medicine 25:2772023SPTBN2 regulates endometroid ovarian cancer cell proliferation, invasion and migration via ITGB4‑mediated focal adhesion and ECM receptor signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLa Yang,Yuanyuan GuPubMed 37206547
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SPTBN2 aa 2300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15020
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Probably plays an important role in neuronal membrane skeleton.
Alternative names
KIAA0302, SCA5, SPTBN2, Beta-III spectrin, Spinocerebellar ataxia 5 protein
Recommended products
Rabbit Polyclonal SPTBN2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SPTBN2 aa 2300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SPTBN2 aa 2300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15020
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Immunoprecipitation - Anti-SPTBN2 antibody (ab264178)
SPTBN2 was immunoprecipitated from 1mg of HeLa cells (20% of IP loaded) using ab264178 at 6 μg per reaction. Western blot was performed on the immunprecipitate using ab264178 at 0.4 μg/ml.
Lane 1: ab264178 in HeLa whole cell lysate
Lane 2: Control IgG in HeLa whole cell lysate
Detected with chemilimuniscence with exposure time of 3 minutes.
Cells prepared using NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-SPTBN2 antibody (ab264178)
Predicted band size: 271 kDa
Western blot - Anti-SPTBN2 antibody (ab264178)
4 - 8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
All lanes: Western blot - Anti-SPTBN2 antibody (ab264178) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HEK-293T whole cell lysate at 50 µg
Lane 4: HEK-293T whole cell lysate at 15 µg
SecondaryAll lanes: Goat anti-Rabbit Light Chain HRP Conjugate
Developed using the ECL technique.
Predicted band size: 271 kDa
Exposure time: 3min
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