Rabbit Recombinant Multiclonal SPTBN2 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPTBN2.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-SPTBN2 Antibody [RP23040132] (AB313451), expandable thumbnail](https://content.abcam.com/products/images/sptbn2-antibody-rp23040132-ab313451--immunocytochemistry-immunofluorescence-img417845.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SPTBN2 Antibody [RP23040132] (AB313451)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- Synthetic Peptide within Human SPTBN2. Database link O15020
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
Target data
Function
Probably plays an important role in neuronal membrane skeleton.
Alternative names
KIAA0302, SCA5, SPTBN2, Beta-III spectrin, Spinocerebellar ataxia 5 protein
Recommended products
Rabbit Recombinant Multiclonal SPTBN2 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SPTBN2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- Synthetic Peptide within Human SPTBN2. Database link O15020
- Clone number
- RP23040132
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-SPTBN2 Antibody [RP23040132] (ab313451), expandable thumbnail](https://content.abcam.com/products/images/sptbn2-antibody-rp23040132-ab313451--immunocytochemistry-immunofluorescence-img417845.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SPTBN2 Antibody [RP23040132] (ab313451)")
Immunocytochemistry/ Immunofluorescence - Anti-SPTBN2 Antibody [RP23040132] (ab313451)
Immunofluorescence was performed on fixed and permeabilized HeLa cells for detection of endogenous Spectrin-Beta3 using ab313451, 2 ug/ml and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor®488 conjugate( 1:2000). Panel a) shows representative cells that were stained for detection and localization of Spectrin-Beta3 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Spectrin-Beta3. Panel e) represents control cells with no primary antibody to assess background.
Downloads
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- Visit the general protocols
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