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AB325666

Anti-SPTBN4 antibody [N393/76] - Chicken IgY (Chimeric)

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Chicken Monoclonal Sptbn4 antibody. Suitable for IHC-P and reacts with Rat samples.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN4 antibody [N393/76] - Chicken IgY (Chimeric) (AB325666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN4 antibody [N393/76] - Chicken IgY (Chimeric) (AB325666)

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by ab325666 at a 1 : 250 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the neuronal body and axon initial segment (AIS) in the cerebral cortex. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN4 antibody [N393/76] - Chicken IgY (Chimeric) (AB325666)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN4 antibody [N393/76] - Chicken IgY (Chimeric) (AB325666)

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by ab325666 at a 1 : 250 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the neuronal body and axon initial segment (AIS) in the cerebral cortex. Magnification 400X.

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

N393/76

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Does not cross-react with other Beta-spectrins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N393/76 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N393/76 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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