AB176706

Anti-SPTLC1 antibody

Name: Anti-SPTLC1 antibody (ab176706) | Abcam
Brand: Abcam Limited
SKU: AB176706

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(10 Publications)

Rabbit Polyclonal SPTLC1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human SPTLC1 aa 50-100.

View Alternative Names

LCB1, SPTLC1, Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, Serine-palmitoyl-CoA transferase 1, LCB 1, SPT 1, SPT1

2 Images

Supplier Data

Immunoprecipitation - Anti-SPTLC1 antibody (AB176706)

ab176706 at 0.4 μg/ml detecting SPTLC1 in HeLa whole cell lysate by WB following IP.

Lane 1 : ab176706 at 6 μg/mg of lysate
Lane 2 : IP with an antibody which recognizes an downstream epitope of SPTLC1.
Lane 3 : Control IgG.

In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SPTLC1 antibody (ab176706)

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-SPTLC1 antibody (AB176706)

All lanes:

Western blot - Anti-SPTLC1 antibody (ab176706) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 53 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SPTLC1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O15269

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176706 was affinity purified using an epitope specific to SPTLC1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core (PubMed : 19416851, PubMed : 33558762, PubMed : 36170811). The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed : 19416851, PubMed : 33558762). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA (PubMed : 19416851, PubMed : 19648650). The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed : 19416851, PubMed : 19648650, PubMed : 33558761, PubMed : 33558762). Required for adipocyte cell viability and metabolic homeostasis (By similarity).

See full target information SPTLC1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40608049

2025

Sphingolipid synthesis maintains nuclear membrane integrity and genome stability during cell division.

Applications

Unspecified application

Species

Unspecified reactive species

Sunyoung Hwang,William Russo,Jaylah Cormier,Jillian Johnson,Sara Martin,Marica Rosaria Ippolito,Sara Cordone,Rui Li,Lihua Julie Zhu,Stefano Santaguida,Eduardo M Torres

Nature 642:776-784 PubMed40269157

2025

Targeting PIKfyve-driven lipid metabolism in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Caleb Cheng,Jing Hu,Rahul Mannan,Tongchen He,Rupam Bhattacharyya,Brian Magnuson,Jasmine P Wisniewski,Sydney Peters,Saadia A Karim,David J MacLean,Hüseyin Karabürk,Li Zhang,Nicholas J Rossiter,Yang Zheng,Lanbo Xiao,Chungen Li,Dominik Awad,Somnath Mahapatra,Yi Bao,Yuping Zhang,Xuhong Cao,Zhen Wang,Rohit Mehra,Pietro Morlacchi,Vaibhav Sahai,Marina Pasca di Magliano,Yatrik M Shah,Lois S Weisman,Jennifer P Morton,Ke Ding,Yuanyuan Qiao,Costas A Lyssiotis,Arul M Chinnaiyan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400794 PubMed39207053

2024

Sphingosine Kinase 2 Regulates Aryl Hydrocarbon Receptor Nuclear Translocation and Target Gene Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Shigetoshi Yokoyama,Imhoi Koo,Daisuke Aibara,Yuan Tian,Iain A Murray,Stephanie L Collins,Denise M Coslo,Mari Kono,Jeffrey M Peters,Richard L Proia,Frank J Gonzalez,Gary H Perdew,Andrew D Patterson

Cell reports 40:111415 PubMed36170811

2022

De novo sphingolipid biosynthesis necessitates detoxification in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meghan E Spears,Namgyu Lee,Sunyoung Hwang,Sung Jin Park,Anne E Carlisle,Rui Li,Mihir B Doshi,Aaron M Armando,Jenny Gao,Karl Simin,Lihua Julie Zhu,Paul L Greer,Oswald Quehenberger,Eduardo M Torres,Dohoon Kim

Journal of lipid research 62:100121 PubMed34560079

2021

Crosstalk between ORMDL3, serine palmitoyltransferase, and 5-lipoxygenase in the sphingolipid and eicosanoid metabolic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Viktor Bugajev,Tomas Paulenda,Pavol Utekal,Michal Mrkacek,Ivana Halova,Ladislav Kuchar,Ondrej Kuda,Petra Vavrova,Björn Schuster,Sergio Fuentes-Liso,Lucie Potuckova,Daniel Smrz,Sara Cernohouzova,Lubica Draberova,Monika Bambouskova,Petr Draber

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33526671

2021

Consequences of aneuploidy in human fibroblasts with trisomy 21.

Applications

Unspecified application

Species

Unspecified reactive species

Sunyoung Hwang,Paola Cavaliere,Rui Li,Lihua Julie Zhu,Noah Dephoure,Eduardo M Torres

International journal of molecular sciences 21: PubMed33171690

2020

Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Elżbieta Supruniuk,Agnieszka Mikłosz,Adrian Chabowski

Cell reports 29:2473-2488.e5 PubMed31747614

2019

Suppressing Aneuploidy-Associated Phenotypes Improves the Fitness of Trisomy 21 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sunyoung Hwang,Jessica F Williams,Maja Kneissig,Maria Lioudyno,Isabel Rivera,Pablo Helguera,Jorge Busciglio,Zuzana Storchova,Megan C King,Eduardo M Torres

Biological chemistry 397:147-55 PubMed26501163

2015

De novo ceramide synthesis is involved in acute inflammation during labor.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Signorelli,Laura Avagliano,Marta R Reforgiato,Nadia Toppi,Josefina Casas,Gemma Fabriàs,Anna Maria Marconi,Riccardo Ghidoni,Anna Caretti

PloS one 9:e85504 PubMed24454878

2014

Dysferlin and other non-red cell proteins accumulate in the red cell membrane of Diamond-Blackfan Anemia patients.

Applications

Species

Human

Esther N Pesciotta,Sira Sriswasdi,Hsin-Yao Tang,David W Speicher,Philip J Mason,Monica Bessler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SPTLC1 antibody

Immunoprecipitation - Anti-SPTLC1 antibody (AB176706)

Western blot - Anti-SPTLC1 antibody (AB176706)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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