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AB237532

Anti-SPTLC3 antibody

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(1 Publication)

Rabbit Polyclonal SPTLC3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SPTLC3 aa 1-100.

View Alternative Names

C20orf38, SPTLC2L, SPTLC3, Serine palmitoyltransferase 3, Long chain base biosynthesis protein 2b, Long chain base biosynthesis protein 3, Serine-palmitoyl-CoA transferase 3, LCB2b, LCB 3, SPT 3

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (AB237532)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (AB237532)

Paraffin-embedded human liver cancer tissue stained for SPTLC3 using ab237532 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (AB237532)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (AB237532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining human colon cancer using ab237532 at 1/100.

Immunocytochemistry/ Immunofluorescence - Anti-SPTLC3 antibody (AB237532)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SPTLC3 antibody (AB237532)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for SPTLC3 (green) using ab237532 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (AB237532)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTLC3 antibody (AB237532)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining human prostate cancer using ab237532 at 1/100.

Western blot - Anti-SPTLC3 antibody (AB237532)
  • WB

Supplier Data

Western blot - Anti-SPTLC3 antibody (AB237532)

All lanes:

Western blot - Anti-SPTLC3 antibody (ab237532) at 1/1000 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate

Lane 4:

Rat heart tissue lysate

Lane 5:

Rat kidney tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 62 kDa

false

Western blot - Anti-SPTLC3 antibody (AB237532)
  • WB

Supplier Data

Western blot - Anti-SPTLC3 antibody (AB237532)

All lanes:

Western blot - Anti-SPTLC3 antibody (ab237532) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate

Lane 2:

Mouse kidney tissue lysate

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SPTLC3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9NUV7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases (PubMed : 19416851, PubMed : 19648650). The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference (PubMed : 19416851). The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference (PubMed : 19416851, PubMed : 19648650).
See full target information SPTLC3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 14:1007-1020 PubMed33603407

2021

Tandem Mass Tag-Based Proteomic Analysis of Potential Biomarkers for Hepatocellular Carcinoma Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Qiang Li,Ge Huang,Bing-Yao Lin,Dongzi Lin,Yan Ma,Zhao Zhang,Tao Chen,Jie Zhou
View all publications
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