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Mouse Recombinant Monoclonal SQSTM1 / p62 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (AB280086), expandable thumbnail
  • Western blot - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (AB280086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] (AB280086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (AB280086), expandable thumbnail
  • Immunoprecipitation - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (AB280086), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWB
Human
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the SQSTM1 gene is an autophagy receptor essential for selective macroautophagy (aggrephagy). It acts as a bridge between polyubiquitinated cargo and autophagosomes, interacting with both the cargo and an autophagy modifier from the MAP1 LC3 family. Alongside WDFY3, it plays a role in forming and autophagically degrading cytoplasmic ubiquitin-containing inclusions and recruiting ubiquitinated proteins to nuclear PML bodies. SQSTM1 may regulate NFKB1 activation by TNF-alpha, nerve growth factor (NGF), and interleukin-1, and may influence titin/TTN signaling in muscle cells. It may also affect signaling cascades via ubiquitination and be involved in cell differentiation, apoptosis, immune response, and regulation of potassium channels. The protein is involved in endosome organization by retaining vesicles in the perinuclear cloud; ubiquitination by RNF26 allows it to attract vesicle-associated adapters, forming a molecular bridge to organize endosomal pathways. Additionally, it promotes the relocalization of Lys-63-linked ubiquitinated STING1 to autophagosomes and acts as an activator of the NFE2L2/NRF2 pathway by interacting with KEAP1, which inactivates the BCR(KEAP1) complex, resulting in nuclear accumulation of NFE2L2/NRF2 and expression of cytoprotective genes. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62

Recommended products

Mouse Recombinant Monoclonal SQSTM1 / p62 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
3/P62 LCK LIGAND
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086), expandable thumbnail

    Western blot - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086)

    SQSTM1 / p62 Western blot staining using mouse Anti-SQSTM1 / p62 antibody

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

    Lanes 1 - 4: Merged signal (red and green). Green - ab280086 observed at 62 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit monoclonal [EPR16891] to GAPDH) observed at 36 kDa.

    Lanes 1-2: ab280086 Anti-SQSTM1/p62 antibody was shown to react with SQSTM1 in HAP1 cells in Western blot. Loss of signal was observed when SQSTM1 knockout sample was used. Wild-type and SQSTM1 knockout samples were subjected to SDS-PAGE. ab280086 and Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated at 4°C overnight at 1/1000 dilution and 1/20000 dilution respectively.

    Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line), whole cell lysate at 20 µg

    Lane 2: SQSTM1 knockout HAP1 (human chronic myelogenous leukemia near-haploid cell line), whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Mouse IgG H&L (IRDye® 800CW) (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Predicted band size: 47 kDa

    Observed band size: 62 kDa

  • Western blot - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086), expandable thumbnail

    Western blot - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086)

    SQSTM1 / p62 Western blot staining using mouse Anti-SQSTM1 / p62 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24086455).

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    Exposure time: 15 seconds

    All lanes: Western blot - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

    Lane 2: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 47 kDa

    Observed band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] (ab280086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] (ab280086)

    Immunohistochemical analysis of paraffin-embedded Human stomach carcinoma tissue labeling SQSTM1 / p62 with ab280086 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human stomach carcinoma. The section was incubated with ab280086 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086)

    SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-SQSTM1 / p62 antibody

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling SQSTM1 / p62 with ab280086 at 1/1000 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human lung carcinoma. The section was incubated with ab280086 for 30 mins at room temperature and followed by mouse specific IgG antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunoprecipitation - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086), expandable thumbnail

    Immunoprecipitation - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086)

    SQSTM1 / p62 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate 10 ug with ab280086 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280086 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate 10 ug

    Lane 2: ab280086 IP in HeLa whole cell lysate

    Lane 3: Mouse monoclonal IgG1(Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control ab18443) instead of ab280086 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 26 seconds

    All lanes: Immunoprecipitation - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] - Autophagosome Marker (ab280086)

    Predicted band size: 47 kDa

    Observed band size: 62 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] (ab280086), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [3/P62 LCK LIGAND] (ab280086)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling SQSTM1 / p62 with ab280086 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)(Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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