Rabbit Polyclonal SQSTM1 / p62 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 73 publications. Immunogen corresponding to Synthetic Peptide within Human SQSTM1 aa 150-300.
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- Haematologica 109:1501-15132024Pharmacologic targeting of the p62 ZZ domain enhances both anti-tumor and bone-anabolic effects of bortezomib in multiple myeloma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia Marino,Daniela N Petrusca,Ryan T Bishop,Judith L Anderson,Hayley M Sabol,Cody Ashby,Justin H Layer,Annamaria Cesarano,Utpal P Davé,Fabiana Perna,Jesus Delgado-Calle,John M Chirgwin,G David RoodmanPubMed 37981834
- Cells 12:2023Exploration of SUMO2/3 Expression Levels and Autophagy Process in Fragile X-Associated Tremor/Ataxia Syndrome: Addressing Study Limitations and Insights for Future Research.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Isabel Alvarez-Mora,Glòria Garrabou,Laura Molina-Porcel,Ruben Grillo-Risco,Francisco Garcia-Garcia,Tamara Barcos,Judith Cantó-Santos,Laia Rodriguez-RevengaPubMed 37830578
- Nature communications 14:42832023An optimized Nurr1 agonist provides disease-modifying effects in Parkinson's disease models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWoori Kim,Mohit Tripathi,Chunhyung Kim,Satyapavan Vardhineni,Young Cha,Shamseer Kulangara Kandi,Melissa Feitosa,Rohit Kholiya,Eric Sah,Anuj Thakur,Yehan Kim,Sanghyeok Ko,Kaiya Bhatia,Sunny Manohar,Young-Bin Kong,Gagandeep Sindhu,Yoon-Seong Kim,Bruce Cohen,Diwan S Rawat,Kwang-Soo KimPubMed 37463889
- Cells 12:2023Extracellular Vesicles Released by Genetically Modified Macrophages Activate Autophagy and Produce Potent Neuroprotection in Mouse Model of Lysosomal Storage Disorder, Batten Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNazira El-Hage,Matthew J Haney,Yuling Zhao,Myosotys Rodriguez,Zhanhong Wu,Mori Liu,Carson J Swain,Hong Yuan,Elena V BatrakovaPubMed 37296618
- International journal of molecular sciences 24:2023Hypothermic Oxygenated Machine Perfusion Promotes Mitophagy Flux against Hypoxia-Ischemic Injury in Rat DCD Liver.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Luo,Yiqing Hu,Yinbiao Qiao,Haoyu Li,Jiacheng Huang,Kangdi Xu,Li Jiang,Hao Wu,Xiaoyi Hu,Junjun Jia,Lin Zhou,Haiyang Xie,Jianhui Li,Shusen ZhengPubMed 36982476
- Frontiers in oncology 12:10618322023New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIoana V Militaru,Alina Adriana Rus,Cristian V A Munteanu,Georgiana Manica,Stefana M PetrescuPubMed 36776379
- Laboratory investigation; a journal of technical methods and pathology 101:837-8502023Extracellular vesicles derived from M2 microglia reduce ischemic brain injury through microRNA-135a-5p/TXNIP/NLRP3 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Liu,You-Ping Li,Li-Min Xiao,Li-Ke Chen,Su-Yue Zheng,Er-Ming Zeng,Chun-Hua XuPubMed 36775555
- Experimental dermatology 32:403-4122022CCL6 promotes M2 polarization and inhibits macrophage autophagy by activating PI3-kinase/Akt signalling pathway during skin wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Feng,Yu Ji,Ce Zhang,Tingting Jin,Jingyu Li,Jincai GuoPubMed 36457234
- Croatian medical journal 63:453-4602022Prognostic relevance of autophagy-related markers p62, LC3, and Beclin1 in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLjubiša Jovanović,Aleksandra Nikolić,Sandra Dragičević,Milena Jović,Radmila JankovićPubMed 36325670
- Anais brasileiros de dermatologia 98:26-352022Inhibition of ANGPT2 activates autophagy during hypertrophic scar formation via PI3K/AKT/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongxin Chen,Kai Xu,Chao Sun,Si Gui,Juanjuan Wu,Song WangPubMed 36272879
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SQSTM1 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13501
Consider this alternative
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
The protein expressed by the SQSTM1 gene is an autophagy receptor essential for selective macroautophagy (aggrephagy). It acts as a bridge between polyubiquitinated cargo and autophagosomes, interacting with both the cargo and an autophagy modifier from the MAP1 LC3 family. Alongside WDFY3, it plays a role in forming and autophagically degrading cytoplasmic ubiquitin-containing inclusions and recruiting ubiquitinated proteins to nuclear PML bodies. SQSTM1 may regulate NFKB1 activation by TNF-alpha, nerve growth factor (NGF), and interleukin-1, and may influence titin/TTN signaling in muscle cells. It may also affect signaling cascades via ubiquitination and be involved in cell differentiation, apoptosis, immune response, and regulation of potassium channels. The protein is involved in endosome organization by retaining vesicles in the perinuclear cloud; ubiquitination by RNF26 allows it to attract vesicle-associated adapters, forming a molecular bridge to organize endosomal pathways. Additionally, it promotes the relocalization of Lys-63-linked ubiquitinated STING1 to autophagosomes and acts as an activator of the NFE2L2/NRF2 pathway by interacting with KEAP1, which inactivates the BCR(KEAP1) complex, resulting in nuclear accumulation of NFE2L2/NRF2 and expression of cytoprotective genes. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62
Recommended products
Rabbit Polyclonal SQSTM1 / p62 antibody. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 73 publications. Immunogen corresponding to Synthetic Peptide within Human SQSTM1 aa 150-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SQSTM1 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13501
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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10 product images
Immunoprecipitation - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686)
Immunoprecipitation of SQSTM1/p62 protein from HeLa whole cell extracts using 5 μg of ab155686.
Western blot analysis was performed using ab155686.
Anti-Rabbit IgG was used as a secondary reagent.All lanes: Immunoprecipitation - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686)
Predicted band size: 47 kDa
Flow Cytometry - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686)
SQSTM1 / p62 Flow Cytometry staining using rabbit Anti-SQSTM1 / p62 antibody
4% paraformaldehyde-fixed HeLa cells stained for SQSTM1/p62 (blue) using ab155686 (1/100) in Flow Cytometry. The cells were fixed at 4°C for 5 minutes. The brown line is unlabelled sample. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
10% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition: Semi-dry, 18 V, 60min (Nitrocellulose membrane).
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: Room temperature for 1 hour.
Washing condition: 5 ml TBST, 4 x 5min.
ECL exposure.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686) at 1/1000 dilution
Lane 1: Untreated (–) HepG2 whole cell extracts at 30 µg
Lane 2: HepG2 treated with 3μM Thapsigargin for 24 hours whole cell extracts (+) at 30 µg
SecondaryAll lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab155686)
Immunocytochemistry/Immunofluorescent analysis of HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) labeling SQSTM1/p62 (green) with ab155686 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde at RT for 15 min. Red: Phalloidin, a F-actin marker.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-SQSTM1 / p62 antibody
Paraffin-embedded human ovarian carcinoma tissue stained for SQSTM1/p62 with ab155686 (1/500) in Immunohistochemical analysis. Antigen retrieval: EDTA based buffer (pH 8) for 15 minutes.
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
10% SDS-PAGE
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686) at 1/500 dilution
Lane 1: HepG2 whole cell extracts at 30 µg
Lane 2: SQSTM1/p62 shRNA transfected HepG2 whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab155686)
ab155686 staining SQSTM1/p62 (autophagosome) in control HeLa cells (left panel) and SQSTM1/p62 in HeLa cells treated with 1uM bafilomycin A1 (Bafilomycin A1, (V)-ATPase inhibitor ab120497) for 18hrs (right panel). The cells were fixed with methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab155686 at 1/500 dilution and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab155686)
Immunocytochemistry/ Immunofluorescence analysis of HepG2 cells treated with 3μM thapsigargin for 12 hours (right) and mock (left) labeling SQSTM1/p62 with ab155686 at 1:500 (green). Cells were fixed in ice-cold MeOH for 10 minutes, permeabilized with cooled acetone for 1 minute. Cells were co-stained with Hoechst (blue).
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
10% SDS-PAGE
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686) at 1/1000 dilution
Lane 1: PC-12 whole cell lysate/extract at 30 µg
Lane 2: Rat2 whole cell lysate/extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686)
SQSTM1 / p62 Western blot staining of A549 whole cell lysate using rabbit Anti-SQSTM1 / p62 antibody
10% SDS PAGE
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab155686) at 1/1000 dilution
All lanes: A549 whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa
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