Name: Anti-SQSTM1 / p62 antibody - Autophagosome Marker
SKU: ab91526
Rating: 4 (6 reviews)

Anti-SQSTM1 / p62 antibody is a rabbit polyclonal antibody that is used to detect SQSTM1 / p62 in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.

- Cited in over 280 publications

Images

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Polyclonal

Immunogen

Synthetic Peptide within Human SQSTM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13501

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF
Human	Tested	Tested	Tested
Mouse	Tested	Tested	Expected
Rat	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 0.50000-2.00000 µg/mL	Notes -
Species Human	Dilution info 0.50000-2.00000 µg/mL	Notes -
Species Rat	Dilution info 0.5-2 µg/mL	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 2.00000-5.00000 µg/mL	Notes -
Species Rat	Dilution info 2.00000-5.00000 µg/mL	Notes -
Species Human	Dilution info 2.00000-5.00000 µg/mL	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

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Target data

See full target information SQSTM1

Function

Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes (PubMed:15340068, PubMed:15953362, PubMed:16286508, PubMed:17580304, PubMed:20168092, PubMed:22017874, PubMed:22622177, PubMed:24128730, PubMed:28404643, PubMed:29343546, PubMed:29507397, PubMed:31857589, PubMed:33509017, PubMed:34471133, PubMed:34893540, PubMed:35831301, PubMed:37306101, PubMed:37802024). Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps (PubMed:16286508, PubMed:20168092, PubMed:22622177, PubMed:24128730, PubMed:28404643, PubMed:29343546, PubMed:29507397, PubMed:34893540, PubMed:37802024). SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies (PubMed:15911346, PubMed:20168092, PubMed:22017874, PubMed:24128730, PubMed:29343546, PubMed:29507397, PubMed:31857589, PubMed:37802024). SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins (PubMed:16286508, PubMed:17580304, PubMed:20168092, PubMed:22622177, PubMed:24128730, PubMed:28404643, PubMed:37802024). SQSTM1 is itself degraded along with its ubiquitinated cargos (PubMed:16286508, PubMed:17580304, PubMed:37802024). Also required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed:20168092). Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes (PubMed:26344566). Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed:20452972, PubMed:28380357, PubMed:33393215, PubMed:37306101). Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes (PubMed:29496741). Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102, PubMed:33472082). Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation (PubMed:25101860). May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 (PubMed:10356400, PubMed:10747026, PubMed:11244088, PubMed:12471037, PubMed:16079148, PubMed:19931284). May play a role in titin/TTN downstream signaling in muscle cells (PubMed:15802564). Adapter that mediates the interaction between TRAF6 and CYLD (By similarity).

Alternative names

ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Polyclonal
Immunogen: Synthetic Peptide within Human SQSTM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13501
Purification technique: Affinity purification Immunogen
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Anti-SQSTM1 / p62 antibody (ab91526) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human samples.

Anti-SQSTM1 / p62 antibody (ab91526) has been cited over 282 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-SQSTM1 / p62 antibody (ab91526) has high sensitivity and specificity.

Anti-SQSTM1 / p62 antibody (ab91526) has 6 independent reviews from customers.

Anti-SQSTM1 / p62 antibody (Anti-ORCA antibody) (ab91526) specifically detects SQSTM1 / p62 (UniProt ID: Q13501; Molecular weight: 48kDa) and is sold in 100 µg selling sizes.

SQSTM1/p62 (Sequestosome 1) is a key protein involved in autophagy, serving as a bridge between ubiquitinated proteins and the autophagic machinery for their degradation. It is essential for maintaining cellular health and responding to stress. Abnormal p62 expression is associated with several diseases, including cancer, where it aids in tumor progression, and neurodegenerative diseases such as Alzheimer's (AD) and Parkinson's disease (PD), where it is involved in the formation of protein aggregates.

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14 product images

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
1 h incubation at RT in 5% NFDM/TBST.
beta-actin was used as a loading control at 1 µg/mL.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 1 µg/mL
Lane 1: HEK293T WT cell lysates at 10 µg
Lane 2: SQSTM1 KO HEK293T cell lysates at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47 kDa
Han, Z. et al PLoS One. 2014 Jan 23;9(1):e86838. doi: 10.1371/journal.pone.0086838. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling SQSTM1 / p62 with ab91526. Heat mediated antigen retrieval was performed. The tissue sections were then blocked with 10% goat serum in PBS, and incubated with primary antibody overnight at 4°C. Sections were incubated with secondary antibody for 1 h at room temperature, incubated with avidin-biotin complex for 1 h at room temperature, rinsed with PBS and then treated with 0.5 mg/mL DAPI.
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
Diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.5 µg/mL
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg
Lane 2: A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg
Lane 3: A549 (human lung carcinoma cell line) cell lysate at 15 µg
Lane 4: CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg
Lane 5: Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Lane 7: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg
Lane 8: K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 15 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg
Lane 10: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg
Lane 11: SK-N-SH (human neuroblastoma cell line) cell lysate at 15 µg
Lane 12: THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg
Lane 13: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg
Lane 14: L1210 (mouse lymphocytic leukemia cell line) cell lysate at 15 µg
Secondary
All lanes: Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Image from Traver MK et al, J Biol Chem. 2011 Sep 2;286(35):30471-80. Epub 2011 Jul 12, Fig 5. DOI 10.1074/jbc.M111.251967 September 2, 2011 The Journal of Biological Chemistry, 286, 30471-30480.
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab91526)
ab91526 at a 1/500 dilution staining SQSTM1/ p62 in wild type murine embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)
Immunofluorescent analysis of 4% paraformaldehyde fixed Mouse Spleen Tissue labeling SQSTM1 / p62 with ab91526 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
Diluting buffer: 5% NFDM/TBST.
Raw 264.7 cells were treated with LPS (0.3 μg/mL) for different time period (0-24 hrs). There was an increase in SQSTM1 protein expression overtime after LPS treatment.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.5 µg/mL
Lane 1: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate - untreated at 15 µg
Lane 2: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 3 hours at 15 µg
Lane 3: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 6 hours at 15 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 24 hours at 15 µg
Secondary
All lanes: Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)
Rat spleen tissue stained for SQSTM1 / p62 using ab91526 at 5 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)
Rat spleen tissue stained for SQSTM1 / p62 using ab91526 at 2 μg/ml in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody (ab91526)
Immunofluorescent analysis of PFA-fixed HeLa cells labeling SQSTM1 with ab91526 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green).
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
1 h incubation at RT in 5% NFDM/TBST
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.2 µg/mL
Lane 1: WT HeLa Cells at 10 µg
Lane 2: SQSTM1 knockout HeLa Cells at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47 kDa
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 1 µg/mL
Lane 1: Mouse brain tissue lysate at 15 µg
Lane 2: Mouse colon tissue lysate at 15 µg
Lane 3: Mouse skeleton muscle tissue lysate at 15 µg
Lane 4: Mouse thymus tissue lysate at 15 µg
Lane 5: Mouse pancreas tissue lysate at 15 µg
Lane 6: Mouse stomach tissue lysate at 15 µg
Predicted band size: 48 kDa
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 1 µg/mL
Lane 1: Rat liver tissue lysate at 15 µg
Lane 2: Rat brain tissue lysate at 15 µg
Lane 3: Rat testis tissue lysate at 15 µg
Lane 4: Rat thymus tissue lysate at 15 µg
Lane 5: Rat pancreas tissue lysate at 15 µg
Lane 6: K562 cell lysate at 15 µg
Secondary
All lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen tissue using rabbit Anti-SQSTM1 / p62 antibody
Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-SQSTM1 antibody (ab91526) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody (ab91526)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human spleen tissue using rabbit Anti-SQSTM1 / p62 antibody
Immunohistochemical analysis of paraffin-embedded Human Spleen Tissue using anti-SQSTM1 antibody (ab91526) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.