Anti-SQSTM1 / p62 antibody is a rabbit polyclonal antibody that is used to detect SQSTM1 / p62 in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 280 publications
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- Nature communications 14:80402023Genes with epigenetic alterations in human pancreatic islets impact mitochondrial function, insulin secretion, and type 2 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTina Rönn,Jones K Ofori,Alexander Perfilyev,Alexander Hamilton,Karolina Pircs,Fabian Eichelmann,Sonia Garcia-Calzon,Alexandros Karagiannopoulos,Hans Stenlund,Anna Wendt,Petr Volkov,Matthias B Schulze,Hindrik Mulder,Lena Eliasson,Sabrina Ruhrmann,Karl Bacos,Charlotte LingPubMed 38086799
- Haematologica 108:3333-33462023Long non-coding RNA mitophagy and ALK-negative anaplastic lymphoma-associated transcript: a novel regulator of mitophagy in T-cell lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValentina Mularoni,Benedetta Donati,Annalisa Tameni,Veronica Manicardi,Francesca Reggiani,Elisabetta Sauta,Magda Zanelli,Marco Tigano,Emanuele Vitale,Federica Torricelli,Stefano Ascani,Giovanni Martino,Giorgio Inghirami,Francesca Sanguedolce,Alessia Ruffini,Alberto Bavieri,Stefano Luminari,Marco Pizzi,Angelo Paolo Dei Tos,Cinzia Fesce,Antonino Neri,Alessia Ciarrocchi,Valentina FragliassoPubMed 37381763
- Journal of orthopaedic surgery and research 18:8922023Exosomal miR-331-3p derived from chemoresistant osteosarcoma cells induces chemoresistance through autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenyang Meng,Yun Yang,Wei Feng,Penglei Ma,Rui BaiPubMed 37993925
- Journal of neuroinflammation 20:2682023Cyanidin-3-O-glucoside protects the brain and improves cognitive function in APPswe/PS1ΔE9 transgenic mice model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Baek, Sanjay,Miey Park,Hae-Jeung LeePubMed 37978414
- iScience 26:1081842023-GlcNAcylation regulates OTX2's proteostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEugenia Wulff-Fuentes,Jeffrey Boakye,Kaeley Kroenke,Rex R Berendt,Carla Martinez-Morant,Michaela Pereckas,John A Hanover,Stephanie Olivier-Van StichelenPubMed 38026167
- Journal of cellular biochemistry 124:1705-17192023The ameliorating effects of mesenchymal stem cells compared to α-tocopherol on apoptosis and autophagy in streptozotocin-induced diabetic rats: Implication of PI3K/Akt signaling pathway and entero-insular axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeba A Mubarak,Manal M Kamal,Yossra Mahmoud,Fatma S Abd-Elsamea,Eman Abdelbary,Marwa G Gamea,Reham I El-MahdyPubMed 37796145
- Molecular medicine reports 28:2023Iron oxide nanoparticles induce ferroptosis via the autophagic pathway by synergistic bundling with paclitaxel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Nie,Wenqing Chen,Tianmei Zhang,Shangrong Ye,Zhongyu Ren,Peng Zhang,Jian WenPubMed 37681444
- Cell death & disease 14:5712023Plk1 promotes renal tubulointerstitial fibrosis by targeting autophagy/lysosome axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Du,Yaqiong Shang,Yun Qian,Yan Guo,Shuang Chen,Xiuli Lin,Weidong Cao,Xiaomei Tang,Anning Zhou,Songming Huang,Aihua Zhang,Zhanjun Jia,Yue ZhangPubMed 37640723
- Journal of cellular physiology 238:2267-22812023Macrophage autophagy contributes to immune liver injury in trichloroethylene sensitized mice: Critical role of TNF-α mediating mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaiwang Ding,Sifan Zhou,Zhoujian Wang,Wei Liu,Lei Gao,Yani Ding,Hua Huang,Qixing Zhu,Jiaxiang ZhangPubMed 37490340
- Brain sciences 13:2023Histone Deacetylase 4 Inhibition Reduces Rotenone-Induced Alpha-Synuclein Accumulation via Autophagy in SH-SY5Y Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuxi Wang,Ling Liu,Chao Han,Haiyang Jiang,Kai Ma,Shiyi Guo,Yun Xia,Fang Wan,Jinsha Huang,Nian Xiong,Tao WangPubMed 37190635
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SQSTM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13501
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Species Rat | Dilution info 0.5-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2.00000-5.00000 µg/mL | Notes - |
Species Rat | Dilution info 2.00000-5.00000 µg/mL | Notes - |
Species Human | Dilution info 2.00000-5.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
The protein expressed by the SQSTM1 gene is an autophagy receptor essential for selective macroautophagy (aggrephagy). It acts as a bridge between polyubiquitinated cargo and autophagosomes, interacting with both the cargo and an autophagy modifier from the MAP1 LC3 family. Alongside WDFY3, it plays a role in forming and autophagically degrading cytoplasmic ubiquitin-containing inclusions and recruiting ubiquitinated proteins to nuclear PML bodies. SQSTM1 may regulate NFKB1 activation by TNF-alpha, nerve growth factor (NGF), and interleukin-1, and may influence titin/TTN signaling in muscle cells. It may also affect signaling cascades via ubiquitination and be involved in cell differentiation, apoptosis, immune response, and regulation of potassium channels. The protein is involved in endosome organization by retaining vesicles in the perinuclear cloud; ubiquitination by RNF26 allows it to attract vesicle-associated adapters, forming a molecular bridge to organize endosomal pathways. Additionally, it promotes the relocalization of Lys-63-linked ubiquitinated STING1 to autophagosomes and acts as an activator of the NFE2L2/NRF2 pathway by interacting with KEAP1, which inactivates the BCR(KEAP1) complex, resulting in nuclear accumulation of NFE2L2/NRF2 and expression of cytoprotective genes. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62
Recommended products
Anti-SQSTM1 / p62 antibody is a rabbit polyclonal antibody that is used to detect SQSTM1 / p62 in ICC/IF, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 280 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SQSTM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q13501
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Anti-SQSTM1 / p62 antibody (ab91526) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human samples.
Anti-SQSTM1 / p62 antibody (ab91526) has been cited over 282 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-SQSTM1 / p62 antibody (ab91526) has high sensitivity and specificity.
Anti-SQSTM1 / p62 antibody (ab91526) has 6 independent reviews from customers.
Anti-SQSTM1 / p62 antibody (Anti-ORCA antibody) (ab91526) specifically detects SQSTM1 / p62 (UniProt ID: Q13501; Molecular weight: 48kDa) and is sold in 100 µg selling sizes.
SQSTM1/p62 (Sequestosome 1) is a key protein involved in autophagy, serving as a bridge between ubiquitinated proteins and the autophagic machinery for their degradation. It is essential for maintaining cellular health and responding to stress. Abnormal p62 expression is associated with several diseases, including cancer, where it aids in tumor progression, and neurodegenerative diseases such as Alzheimer's (AD) and Parkinson's disease (PD), where it is involved in the formation of protein aggregates.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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14 product images
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
1 h incubation at RT in 5% NFDM/TBST.
beta-actin was used as a loading control at 1 µg/mL.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 1 µg/mL
Lane 1: HEK293T WT cell lysates at 10 µg
Lane 2: SQSTM1 KO HEK293T cell lysates at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47 kDa
Han, Z. et al PLoS One. 2014 Jan 23;9(1):e86838. doi: 10.1371/journal.pone.0086838. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-SQSTM1 / p62 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling SQSTM1 / p62 with ab91526. Heat mediated antigen retrieval was performed. The tissue sections were then blocked with 10% goat serum in PBS, and incubated with primary antibody overnight at 4°C. Sections were incubated with secondary antibody for 1 h at room temperature, incubated with avidin-biotin complex for 1 h at room temperature, rinsed with PBS and then treated with 0.5 mg/mL DAPI.
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
Diluting buffer: 5% NFDM/TBST.All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.5 µg/mL
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg
Lane 2: A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg
Lane 3: A549 (human lung carcinoma cell line) cell lysate at 15 µg
Lane 4: CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg
Lane 5: Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg
Lane 6: HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Lane 7: HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg
Lane 8: K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 15 µg
Lane 9: MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg
Lane 10: Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg
Lane 11: SK-N-SH (human neuroblastoma cell line) cell lysate at 15 µg
Lane 12: THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg
Lane 13: NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg
Lane 14: L1210 (mouse lymphocytic leukemia cell line) cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Image from Traver MK et al, J Biol Chem. 2011 Sep 2;286(35):30471-80. Epub 2011 Jul 12, Fig 5. DOI 10.1074/jbc.M111.251967 September 2, 2011 The Journal of Biological Chemistry, 286, 30471-30480.Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-SQSTM1 / p62 antibody
ab91526 at a 1/500 dilution staining SQSTM1/ p62 in wild type murine embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-SQSTM1 / p62 antibody
Immunofluorescent analysis of 4% paraformaldehyde fixed Mouse Spleen Tissue labeling SQSTM1 / p62 with ab91526 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
Diluting buffer: 5% NFDM/TBST.Raw 264.7 cells were treated with LPS (0.3 μg/mL) for different time period (0-24 hrs). There was an increase in SQSTM1 protein expression overtime after LPS treatment.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.5 µg/mL
Lane 1: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate - untreated at 15 µg
Lane 2: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 3 hours at 15 µg
Lane 3: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 6 hours at 15 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 24 hours at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-SQSTM1 / p62 antibody
Rat spleen tissue stained for SQSTM1 / p62 using ab91526 at 5 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-SQSTM1 / p62 antibody
Rat spleen tissue stained for SQSTM1 / p62 using ab91526 at 2 μg/ml in immunohistochemical analysis.
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 1 µg/mL
Lane 1: Rat liver tissue lysate at 15 µg
Lane 2: Rat brain tissue lysate at 15 µg
Lane 3: Rat testis tissue lysate at 15 µg
Lane 4: Rat thymus tissue lysate at 15 µg
Lane 5: Rat pancreas tissue lysate at 15 µg
Lane 6: K562 cell lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human spleen tissue using rabbit Anti-SQSTM1 / p62 antibody
Immunohistochemical analysis of paraffin-embedded Human Spleen Tissue using anti-SQSTM1 antibody (ab91526) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen tissue using rabbit Anti-SQSTM1 / p62 antibody
Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-SQSTM1 antibody (ab91526) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
1 h incubation at RT in 5% NFDM/TBST
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.2 µg/mL
Lane 1: WT HeLa Cells at 10 µg
Lane 2: SQSTM1 knockout HeLa Cells at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47 kDa
Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Western blot staining using rabbit Anti-SQSTM1 / p62 antibody
1 h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 1 µg/mL
Lane 1: Mouse brain tissue lysate at 15 µg
Lane 2: Mouse colon tissue lysate at 15 µg
Lane 3: Mouse skeleton muscle tissue lysate at 15 µg
Lane 4: Mouse thymus tissue lysate at 15 µg
Lane 5: Mouse pancreas tissue lysate at 15 µg
Lane 6: Mouse stomach tissue lysate at 15 µg
Predicted band size: 48 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526)
SQSTM1 / p62 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-SQSTM1 / p62 antibody
Immunofluorescent analysis of PFA-fixed HeLa cells labeling SQSTM1 with ab91526 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green).
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