Anti-SQSTM1 / p62 antibody - Autophagosome Marker

• WB

Supplier Data

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

1 h incubation at RT in 5% NFDM/TBST.

beta-actin was used as a loading control at 1 µg/mL.

All lanes:

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 1 µg/mL

Lane 1:

HEK293T WT cell lysates at 10 µg

Lane 2:

SQSTM1 KO HEK293T cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 47 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Immunohistochemical analysis of paraffin-embedded Human Spleen Tissue using anti-SQSTM1 antibody (ab91526) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Immunofluorescent analysis of PFA-fixed HeLa cells labeling SQSTM1 with ab91526 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Rat spleen tissue stained for SQSTM1 / p62 using ab91526 at 5 μg/ml in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Immunofluorescent analysis of 4% paraformaldehyde fixed Mouse Spleen Tissue labeling SQSTM1 / p62 with ab91526 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Rat spleen tissue stained for SQSTM1 / p62 using ab91526 at 2 μg/ml in immunohistochemical analysis.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling SQSTM1 / p62 with ab91526. Heat mediated antigen retrieval was performed. The tissue sections were then blocked with 10% goat serum in PBS, and incubated with primary antibody overnight at 4°C. Sections were incubated with secondary antibody for 1 h at room temperature, incubated with avidin-biotin complex for 1 h at room temperature, rinsed with PBS and then treated with 0.5 mg/mL DAPI.

Han, Z. et al PLoS One. 2014 Jan 23;9(1):e86838. doi: 10.1371/journal.pone.0086838. eCollection 2014 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

ab91526 at a 1/500 dilution staining SQSTM1/ p62 in wild type murine embryonic fibroblasts by Immunocytochemistry/ Immunofluorescence.

Image from Traver MK et al, J Biol Chem. 2011 Sep 2;286(35):30471-80. Epub 2011 Jul 12, Fig 5. DOI 10.1074/jbc.M111.251967 September 2, 2011 The Journal of Biological Chemistry, 286, 30471-30480.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-SQSTM1 antibody (ab91526) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.5 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 15 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) cell lysate at 15 µg

Lane 3:

A549 (human lung carcinoma cell line) cell lysate at 15 µg

Lane 4:

CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate at 15 µg

Lane 5:

Daudi (human Burkitt's lymphoma cell line) cell lysate at 15 µg

Lane 6:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

Lane 7:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 15 µg

Lane 8:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 15 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 15 µg

Lane 10:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 15 µg

Lane 11:

SK-N-SH (human neuroblastoma cell line) cell lysate at 15 µg

Lane 12:

THP-1 (human monocytic leukemia cell line) cell lysate at 15 µg

Lane 13:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 15 µg

Lane 14:

L1210 (mouse lymphocytic leukemia cell line) cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

1 h incubation at RT in 5% NFDM/TBST

All lanes:

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.2 µg/mL

Lane 1:

WT HeLa Cells at 10 µg

Lane 2:

SQSTM1 knockout HeLa Cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (AB91526)

Diluting buffer : 5% NFDM/TBST.

Raw 264.7 cells were treated with LPS (0.3 μg/mL) for different time period (0-24 hrs). There was an increase in SQSTM1 protein expression overtime after LPS treatment.

All lanes:

Western blot - Anti-SQSTM1 / p62 antibody - Autophagosome Marker (ab91526) at 0.5 µg/mL

Lane 1:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate - untreated at 15 µg

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 3 hours at 15 µg

Lane 3:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 6 hours at 15 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate treated with 0.3 μg/mL LPS for 24 hours at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 47 kDa

false