Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free

• ICC

Lab

Immunocytochemistry - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

This data was developed using the same antibody clone in a different buffer formulation (ab240635). ab240635 staining SQSTM1 in wild-type Hap1 cells and SQSTM1 knockout Hap1 cells treated with chloroquine (ab142116, 50μM for 24 hrs). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab240635 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor^® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

This data was developed using the same antibody clone in a different buffer formulation (ab240635). ab240635 was shown to react with SQSTM1 in wild-type U-2 OS cells in Immunocytochemistry with loss of signal observed in a SQSTM1 knockout cell line. Wild-type and knockout cells were mixed and pelleted at a 1 : 1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1/5000. The cells were then incubated with ab240635 at 1/500 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor^® 555) at 0.5 μg/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880). These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SQSTM1 / p62 with ab240635 at 1/50 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240635).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SQSTM1 / p62 with ab240635 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240635).

• IP

Unknown

Immunoprecipitation - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

SQSTM1 / p62 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab240635 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240635 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10ug

Lane 2 : ab240635 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab240635 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240635).

All lanes:

Immunoprecipitation - Anti-SQSTM1 / p62 antibody [EPR23101-103] - Autophagosome Marker (<a href='/en-us/products/primary-antibodies/sqstm1-p62-antibody-epr23101-103-autophagosome-marker-ab240635'>ab240635</a>)

Predicted band size: 47 kDa

Observed band size: 62 kDa

false

• IP

Collaborator

Immunoprecipitation - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

This data was developed using the same antibody clone in a different buffer formulation (ab269458). Immunoprecipitation of SQSTM1 in U-2 OS cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab240635 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab56416 at 1/5000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Immunoprecipitation - Anti-SQSTM1 / p62 antibody [EPR23101-103] - Autophagosome Marker (<a href='/en-us/products/primary-antibodies/sqstm1-p62-antibody-epr23101-103-autophagosome-marker-ab240635'>ab240635</a>)

Predicted band size: 47 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (Mouse embryo fibroblast) cells labelling SQSTM1 / p62 with ab240635 at 1/50 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MEF cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240635).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MEF (Mouse embryonic fibroblast (immortalized)) cells labelling SQSTM1 / p62 with ab240635 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240635).

• IP

Unknown

Immunoprecipitation - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

SQSTM1 / p62 was immunoprecipitated from 0.35 mg MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate with ab240635 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240635 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : MEF whole cell lysate 10ug

Lane 2 : ab240635 IP in MEF whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab240635 in MEF whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 min.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240635).

All lanes:

Immunoprecipitation - Anti-SQSTM1 / p62 antibody [EPR23101-103] - Autophagosome Marker (<a href='/en-us/products/primary-antibodies/sqstm1-p62-antibody-epr23101-103-autophagosome-marker-ab240635'>ab240635</a>)

Predicted band size: 47 kDa

Observed band size: 62 kDa

false

• WB

Collaborator

Western blot - Anti-SQSTM1 / p62 antibody [EPR23101-103] - BSA and Azide free (AB269458)

This data was developed using the same antibody in a different buffer formulation (ab240635).

ab240635 was shown to react with SQSTM1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a SQSTM1 knockout cell line. Wild-type U-2 OS and SQSTM1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab240635 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-SQSTM1 / p62 antibody [EPR23101-103] - Autophagosome Marker (<a href='/en-us/products/primary-antibodies/sqstm1-p62-antibody-epr23101-103-autophagosome-marker-ab240635'>ab240635</a>) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

SQSTM1 knockout U-2 OS cell lysate at 20 µg

Predicted band size: 47 kDa

false