Goat Recombinant Monoclonal SQSTM1 / p62 antibody. Carrier free. Suitable for ICC/IF and reacts with Human, Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (AB323250), expandable thumbnail](https://content.abcam.com/products/images/sqstm1-p62-antibody-epr4844-goat-igg-chimeric-bsa-and-azide-free-ab323250--immunocytochemistry-immunofluorescence-img440841.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (AB323250)")
![Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (AB323250), expandable thumbnail](https://content.abcam.com/products/images/sqstm1-p62-antibody-epr4844-goat-igg-chimeric-bsa-and-azide-free-ab323250--immunocytochemistry-immunofluorescence-img440842.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (AB323250)")
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
The protein expressed by the SQSTM1 gene is an autophagy receptor essential for selective macroautophagy (aggrephagy). It acts as a bridge between polyubiquitinated cargo and autophagosomes, interacting with both the cargo and an autophagy modifier from the MAP1 LC3 family. Alongside WDFY3, it plays a role in forming and autophagically degrading cytoplasmic ubiquitin-containing inclusions and recruiting ubiquitinated proteins to nuclear PML bodies. SQSTM1 may regulate NFKB1 activation by TNF-alpha, nerve growth factor (NGF), and interleukin-1, and may influence titin/TTN signaling in muscle cells. It may also affect signaling cascades via ubiquitination and be involved in cell differentiation, apoptosis, immune response, and regulation of potassium channels. The protein is involved in endosome organization by retaining vesicles in the perinuclear cloud; ubiquitination by RNF26 allows it to attract vesicle-associated adapters, forming a molecular bridge to organize endosomal pathways. Additionally, it promotes the relocalization of Lys-63-linked ubiquitinated STING1 to autophagosomes and acts as an activator of the NFE2L2/NRF2 pathway by interacting with KEAP1, which inactivates the BCR(KEAP1) complex, resulting in nuclear accumulation of NFE2L2/NRF2 and expression of cytoprotective genes. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62
Goat Recombinant Monoclonal SQSTM1 / p62 antibody. Carrier free. Suitable for ICC/IF and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR4844
- Purification technique
- Affinity purification Protein G
- Specificity
This antibody got too weak band in rat tissues. you may need to optimize experimental protocols (increasing lysate amount. using lower dilution or higher sensitivity ECL substrate) to get visible band. However it performs very well in rat cell lines.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker ab109012). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
ab323250 is the carrier-free version of Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker – Goat IgG (Chimeric) ab323241
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (ab323250), expandable thumbnail](https://content.abcam.com/products/images/sqstm1-p62-antibody-epr4844-goat-igg-chimeric-bsa-and-azide-free-ab323250--immunocytochemistry-immunofluorescence-img440841.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (ab323250)")
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (ab323250)
Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker – Goat IgG (Chimeric) ab323241 staining SQSTM1 in wild-type Hap1 cells and SQSTM1 knockout Hap1 cells +/- Chloroquine (50uM, 24 hours) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal donkey serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker – Goat IgG (Chimeric) ab323241 at 5ug/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed ab150133, Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This data was developed using the same antibody clone in a different buffer formulation.
![Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (ab323250), expandable thumbnail](https://content.abcam.com/products/images/sqstm1-p62-antibody-epr4844-goat-igg-chimeric-bsa-and-azide-free-ab323250--immunocytochemistry-immunofluorescence-img440842.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (ab323250)")
Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR4844] - Goat IgG (Chimeric) – BSA and Azide Free (ab323250)
Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker – Goat IgG (Chimeric) ab323241 staining SQSTM1 in NIH/3T3 +/- chloroquine (50uM, 24 hours) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal donkey serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4° C with Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker – Goat IgG (Chimeric) ab323241 at 1ug/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed ab150133, Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
This data was developed using the same antibody clone in a different buffer formulation.
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