Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free (AB317629)

This data was developed using ab317628, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (<a href='/en-us/products/primary-antibodies/squalene-epoxidase-antibody-epr29195-89-ab317628'>ab317628</a>) at 1/1000 dilution

All lanes:

Human liver cancer tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 58 kDa,64 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free (AB317629)

This data was developed using ab317628, the same antibody clone in a different buffer formulation.

Low expression : KARPAS-299, SU-DHL-1 (PMID : 30760928).

The molecular weight observed is consistent with what has been described in the literature (PMID : 33933449).

The identity of the bands higher than 75 kDa and lower than 45 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 59 seconds; Lanes 4-6 : 158 seconds; Lanes 7-8 : 59 seconds; Lane 9 : 125 seconds.

All lanes:

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (<a href='/en-us/products/primary-antibodies/squalene-epoxidase-antibody-epr29195-89-ab317628'>ab317628</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

KARPAS-299 (human t cell lymphoma cell) whole cell lysate at 20 µg

Lane 3:

SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

Lane 6:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 8:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 9:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 53 kDa,58 kDa,64 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free (AB317629)

This data was developed using ab317628, the same antibody clone in a different buffer formulation.

The identity of the bands between 25 kDa and 37 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (<a href='/en-us/products/primary-antibodies/squalene-epoxidase-antibody-epr29195-89-ab317628'>ab317628</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

F9 transfected with siRNA specifically targeting Squalene Epoxidase whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 64 kDa,36 kDa

false

Exposure time: 62s

• WB

Supplier Data

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free (AB317629)

This data was developed using ab317628, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (<a href='/en-us/products/primary-antibodies/squalene-epoxidase-antibody-epr29195-89-ab317628'>ab317628</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

EL4 (mouse lymphoma t lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 64 kDa

false

Exposure time: 180s