Anti-SR1 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR1 antibody (AB71983)

Paraffin embedded human small intestine tissue immunostained using ab71983 at 10 μg/ml.

• WB

Unknown

Western blot - Anti-SR1 antibody (AB71983)

All lanes:

Western blot - Anti-SR1 antibody (ab71983) at 1 µg/mL

Lane 1:

human brain lysate

Lane 2:

human brain lysate with immunising peptide

Lane 3:

mouse brain lysate

Lane 4:

rat brain lysate

Predicted band size: 21 kDa

Observed band size: 22 kDa

false

• WB

Lab

Western blot - Anti-SR1 antibody (AB71983)

Western blot : Anti-SR1 antibody ab71983 staining at 1 µg/mL, shown in black; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 22 kDa in Wild-type A549 cell lysates with no signal observed at this size in SRI knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SR1 antibody (ab71983) at 1 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

SRI knockout A549 at 20 µg

Lane 3:

PC-3 at 20 µg

Lane 4:

HepG2 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit HRP (H+L) at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

true

Exposure time: 60s