Rabbit Polyclonal SR140 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human U2SURP aa 1-50.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human U2SURP aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O15042
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Sheep | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Alternative names
U2 snRNP-associated SURP motif-containing protein, 140 kDa Ser/Arg-rich domain protein, U2-associated protein SR140, SR140, KIAA0332, U2SURP
Recommended products
Rabbit Polyclonal SR140 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human U2SURP aa 1-50.
Alternative names
U2 snRNP-associated SURP motif-containing protein, 140 kDa Ser/Arg-rich domain protein, U2-associated protein SR140, SR140, KIAA0332, U2SURP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human U2SURP aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O15042
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab195340 was affinity purified using an epitope specific to SR140 immobilized on a solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-SR140 antibody (ab195340)
All lanes: Western blot - Anti-SR140 antibody (ab195340) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate prepared using NETN lysis buffer at 50 µg
Lane 2: 293T whole cell lysate prepared using NETN lysis buffer at 50 µg
Lane 3: Jurkat whole cell lysate prepared using NETN lysis buffer at 50 µg
Lane 4: Mouse TCMK-1 whole cell lysate prepared using NETN lysis buffer at 50 µg
Lane 5: Mouse NIH/3T3 whole cell lysate prepared using NETN lysis buffer at 50 µg
Developed using the ECL technique.
Predicted band size: 118 kDa
Exposure time: 30s
Immunoprecipitation - Anti-SR140 antibody (ab195340)
Detection of SR140 in Immunoprecipitates of 293T whole cell lysates prepared
using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab195340 at 6 μg per reaction for IP and at 0.1μg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.All lanes: Immunoprecipitation - Anti-SR140 antibody (ab195340)
Predicted band size: 118 kDa
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