AB62516

Anti-sRANKL antibody

Name: Anti-sRANKL antibody (ab62516) | Abcam
Brand: Abcam Limited
SKU: AB62516
Price: 585 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(27 Publications)

Rabbit Polyclonal RANKL antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF11.

View Alternative Names

CD254, OPGL, RANKL, TRANCE, TNFSF11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sRANKL antibody (AB62516)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sRANKL antibody (AB62516)

Immunohistochemical analysis of paraffin-embedded human liver tissue using ab62516 at 5μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4°C. Goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as a secondary. Counter stained with Hematoxylin.

Supplier Data

Western blot - Anti-sRANKL antibody (AB62516)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-sRANKL antibody (ab62516) at 1 µg/mL

Lane 1:

A431 cell lysate at 15 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa

false

Unknown

Western blot - Anti-sRANKL antibody (AB62516)

Blocking buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-sRANKL antibody (ab62516) at 0.25 µg/mL

Lane 2:

Western blot - Anti-sRANKL antibody (ab62516) at 0.5 µg/mL

All lanes:

Rat liver tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 35 kDa

Observed band size: 30 kDa

false

Supplier Data

Western blot - Anti-sRANKL antibody (AB62516)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-sRANKL antibody (ab62516) at 2 µg/mL

Lane 1:

Mouse kidney tissue lysate at 15 µg

Lane 2:

Mouse skin tissue lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TNFSF11. The exact immunogen used to generate this antibody is proprietary information.

O14788

Specificity

IHC-P has not been validated for use with mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "Only validated for human samples." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-0.5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SRANKL also known as soluble receptor activator of nuclear factor kappa-B ligand is an essential protein involved in bone metabolism. It acts as a cytokine and is a member of the tumor necrosis factor (TNF) superfamily. sRANKL is derived from the membrane-bound form of RANKL by proteolytic cleavage and has a molecular weight of approximately 18 kDa. This protein is predominantly found in osteoblasts osteocytes and T-cells where it plays a critical role in physiological processes.

Biological function summary

SRANKL facilitates the differentiation and activation of osteoclasts which are cells responsible for bone resorption. It binds to its receptor RANK on osteoclast precursors promoting their maturation into fully functional osteoclasts. This binding process involves complex formation and interaction with another molecule called osteoprotegerin (OPG) which acts as a decoy receptor regulating sRANKL activity and therefore controlling bone resorption.

Pathways

SRANKL plays a fundamental role in the RANK/RANKL/OPG signaling pathway which is important for maintaining bone homeostasis. This pathway regulates the balance between bone formation and resorption. It also interacts with NF-kappaB signaling a pathway that influences inflammation and immune response. sRANKL through these pathways closely connects with proteins such as TRAF6 which mediates downstream signaling leading to osteoclastogenesis.

Alterations in the sRANKL pathway impact bone-related diseases such as osteoporosis and rheumatoid arthritis. In osteoporosis increased levels of sRANKL enhance osteoclast activity leading to excessive bone resorption and thereby weakening bones. In rheumatoid arthritis the interaction between sRANKL and RANK promotes inflammation and joint destruction. This protein is linked to OPG as variations in their ratio influence disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed : 22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

See full target information TNFSF11

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 16:1630268 PubMed40704286

2025

Metamizole does not affect fracture healing in a murine ischemia model.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Schönbeck,Janine Stutz,Sebastian T Schreiber,Lukas Keller,Chiara Siep,Wolfgang Metzger,Mario Giorgi,Irene Sartini,Tobias Fritz,Tim Pohlemann,Michael D Menger,Emmanouil Liodakis,Matthias W Laschke,Marcel Orth

Nutrients 17: PubMed40573160

2025

High Dietary Phosphorus Impairs Bone Microarchitecture and Induces Alterations in the LGR4-R-Spondins Axis in Rats with Normal Renal Function.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Fernández-Villabrille,Francisco Baena-Huerta,Laura Suárez-Fernández,Elena Nefyodova,Paula Calvó,Nerea González-García,Helena Gil-Peña,Carlos Gómez-Alonso,Cristina Alonso-Montes,Manuel Naves-Díaz,Christa Maes,Natalia Carrillo-López,Sara Panizo

Biomedicines 12: PubMed39595193

2024

Chamomile Tincture and Lidocaine Hydrochloride Gel Ameliorates Periodontitis: A Preclinical Study.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Sun,Huiyi Wang,Junhong Xiao,Qiudong Yang,Heyu Liu,Zhengkun Yang,Yuqi Liu,Xin Huang,Liu Yang,Li Ma,Zhengguo Cao

Advanced healthcare materials 13:e2402350 PubMed39370575

2024

Development of a Microfluidic Vascularized Osteochondral Model as a Drug Testing Platform for Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Shima Salehi,Stefania Brambilla,Marco Rasponi,Silvia Lopa,Matteo Moretti

Evidence-based complementary and alternative medicine : eCAM 2023:4167906 PubMed37123081

2023

Iridoid from Oliv. Exerts Antiarthritis Effects by Inhibiting the JAK2/STAT3 Signaling Pathway and .

Applications

Unspecified application

Species

Unspecified reactive species

Li-Dong Tang,Jian-Ying Wang,Yan Zhang,Xiao-Yun Chen,Lei Zhang,Ying Yuan

International journal of dentistry 2022:7398924 PubMed36794024

2022

Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Fumie Shiba,Hisako Furusho,Takashi Takata,Rika Shimizu,Mutsumi Miyauchi

JCI insight 7: PubMed36173680

2022

Increased joint loading induces subchondral bone loss of the temporomandibular joint via the RANTES-CCRs-Akt2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Yang Feng,Jie Lei,Yu-Xiang Li,Wen-Ge Shi,Ran-Ran Wang,Adrian Ujin Yap,Yi-Xiang Wang,Kai-Yuan Fu

Journal of periodontology 94:244-255 PubMed35892139

2022

Abaloparatide outperforms teriparatide in protecting against alveolar bone loss in experimental periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Yuran Qian,Hui Qiao,Weiyi Pan,Liang Xie,Yu Li

Iranian journal of basic medical sciences 23:1315-1322 PubMed33149864

2020

Orthodontic treatment induces Th17/Treg cells to regulate tooth movement in rats with periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Ge,Jing Peng,Lan Yu,Shuo Huang,Lu Xu,Ying Su,Li Chen

Molecular medicine reports 22:3453-3463 PubMed32945471

2020

Mechanisms by which electroacupuncture‑mediated histone acetylation mitigates bone loss in rats with ovariectomy‑induced osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Shu,Yuwei Shao,Ruolan Liu,Yan Hu,Zhao Peng,Jun Tian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-sRANKL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-sRANKL antibody (AB62516)

Western blot - Anti-sRANKL antibody (AB62516)

Western blot - Anti-sRANKL antibody (AB62516)

Western blot - Anti-sRANKL antibody (AB62516)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (27)

Product promise