Rabbit Recombinant Monoclonal LYN phospho Y418 antibody. Carrier free. Suitable for ELISA, WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Rat, Human, Mouse samples.
Images
![Flow Cytometry (Intracellular) - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (AB239824), expandable thumbnail](https://content.abcam.com/products/images/src-family-phospho-y418-antibody-ep503y-bsa-and-azide-free-ab239824--flow-cytometry-intracellular-img112900.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (AB239824)")
![Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (AB239824), expandable thumbnail](https://content.abcam.com/products/images/src-family-phospho-y418-antibody-ep503y-bsa-and-azide-free-ab239824--immunocytochemistry-immunofluorescence-img91266.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (AB239824)")
![ELISA - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (AB239824), expandable thumbnail](https://content.abcam.com/products/images/src-family-phospho-y418-antibody-ep503y-bsa-and-azide-free-ab239824--elisa-img104099.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (AB239824)")
![Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (AB239824), expandable thumbnail](https://content.abcam.com/products/images/src-family-phospho-y418-antibody-ep503y-bsa-and-azide-free-ab239824--immunocytochemistry-immunofluorescence-img28802.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (AB239824)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ELISA | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Predicted | Expected | Expected | Expected |
| Rat | Expected | Tested | Tested | Expected |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Induction of cells with Pervanadate: Prepare 500mM stock Sodium Orthovanadate in PBS. To make 100mM (100x) Pervanadate Na3VO4 right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2, vortex and incubate at room RT for 5-10min. Cells are serum-starved overnight, and then treated with freshly made Pervanadate (100x, final concentration 1mM) for 20min at 37°C. |
Species Human | Dilution info - | Notes Induction of cells with Pervanadate: Prepare 500mM stock Sodium Orthovanadate in PBS. To make 100mM (100x) Pervanadate Na3VO4 right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2, vortex and incubate at room RT for 5-10min. Cells are serum-starved overnight, and then treated with freshly made Pervanadate (100x, final concentration 1mM) for 20min at 37°C. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Induction of cells with Pervanadate: Prepare 500mM stock Sodium Orthovanadate in PBS. To make 100mM (100x) Pervanadate Na3VO4 right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2, vortex and incubate at room RT for 5-10min. Cells are serum-starved overnight, and then treated with freshly made Pervanadate (100x, final concentration 1mM) for 20min at 37°C. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Version
Target data
Function
Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down-regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Involved in the regulation of endothelial activation, neutrophil adhesion and transendothelial migration (PubMed:36932076). Down-regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Mediates phosphorylation of the BCR-ABL fusion protein. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR (erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. Regulates phosphatidylinositol 3-kinase activity and AKT1 activation. Regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr-72'. Kinase activity facilitates TLR4-TLR6 heterodimerization and signal initiation. Phosphorylates SCIMP on 'Tyr-107'; this enhances binding of SCIMP to TLR4, promoting the phosphorylation of TLR4, and a selective cytokine response to lipopolysaccharide in macrophages (By similarity). Phosphorylates CLNK (By similarity). Phosphorylates BCAR1/CAS and NEDD9/HEF1 (PubMed:9020138).
Alternative names
JTK8, LYN, Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn
Recommended products
Rabbit Recombinant Monoclonal LYN phospho Y418 antibody. Carrier free. Suitable for ELISA, WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Rat, Human, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP503Y
- Purification technique
Affinity purification Protein A
- Specificity
This antibody recognises Lyn phosphorylation on Tyrosine 396.BLAST analysis show 100% homology with Hck (pY410) and Lck (pY393), and 92% homology with Src (pY418), Fyn (pY419) and Yes (pY425) Therefore, the antibody may cross-react with these proteins.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab239824 is the carrier-free version of Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660.
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry (Intracellular) - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824), expandable thumbnail](https://content.abcam.com/products/images/src-family-phospho-y418-antibody-ep503y-bsa-and-azide-free-ab239824--flow-cytometry-intracellular-img112900.jpg "Flow Cytometry (Intracellular) - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)")
Flow Cytometry (Intracellular) - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)
Intracellular Flow Cytometry analysis of HeLa cells (treated with 1mM pervanadate for 30 minutes) labelling SRC Family (phospho Y418) with purified Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660 at 1/80 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody.
Black - Isotype control, rabbit monoclonal IgG with pervanade treated HeLa cells.
Blue - Unlabelled control, pervanadate treated HeLa cells without incubation with primary and secondary antibodies.
Red - Pervandate treated HeLa cells with Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660.
Green - Untreated HeLa cells labelled with Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660).
![Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824), expandable thumbnail](https://content.abcam.com/products/images/src-family-phospho-y418-antibody-ep503y-bsa-and-azide-free-ab239824--immunocytochemistry-immunofluorescence-img91266.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)")
Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells (untreated and treated with 1mM pervanadate for 15 minutes) labelling SRC Family (phospho Y418) with purified Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660).
![ELISA - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824), expandable thumbnail](https://content.abcam.com/products/images/src-family-phospho-y418-antibody-ep503y-bsa-and-azide-free-ab239824--elisa-img104099.jpg "ELISA - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)")
ELISA - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)
Direct ELISA antibody dose-response curve using unpurified Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660 at 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660).
![Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824), expandable thumbnail](https://content.abcam.com/products/images/src-family-phospho-y418-antibody-ep503y-bsa-and-azide-free-ab239824--immunocytochemistry-immunofluorescence-img28802.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)")
Immunocytochemistry/ Immunofluorescence - Anti-SRC Family (phospho Y418) antibody [EP503Y] - BSA and Azide free (ab239824)
ICC/IF image of unpurified Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SRC Family (phospho Y418) antibody [EP503Y] ab40660).
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