AB10310

Anti-SRC3 antibody

Name: Anti-SRC3 antibody (ab10310) | Abcam
Brand: Abcam Limited
SKU: AB10310
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal SRC3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NCOA3 aa 900-950.

View Alternative Names

AIB1, BHLHE42, RAC3, TRAM1, NCOA3, Nuclear receptor coactivator 3, NCoA-3, ACTR, Amplified in breast cancer 1 protein, CBP-interacting protein, Class E basic helix-loop-helix protein 42, Receptor-associated coactivator 3, Steroid receptor coactivator protein 3, Thyroid hormone receptor activator molecule 1, AIB-1, pCIP, bHLHe42, RAC-3, SRC-3, TRAM-1

2 Images

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Immunoprecipitation - Anti-SRC3 antibody (AB10310)

Detection of SCR3 by Immunoprecipitation.

Samples : Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 μg/ml extract. Detection : Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.

All lanes:

Immunoprecipitation - Anti-SRC3 antibody (ab10310)

Predicted band size: 155 kDa

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Unknown

Western blot - Anti-SRC3 antibody (AB10310)

Detection of SRC3 by Western Blot.

Samples : Nuclear extract (5 μg) from HeLa cells. Primary antibody ab10310 used at 0.33 μg/ml. Detection : Alkaline phosphatase conjugated goat anti-rabbit IgG diluted 1/3000 with NBT-BCIP as substrate for 20 min.

Detection of SCR3 by Immunoprecipitation. Samples : Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 µg/ml extract. Detection : Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.

All lanes:

Western blot - Anti-SRC3 antibody (ab10310)

Predicted band size: 155 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human NCOA3 aa 900-950. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6Q9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. SRC3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

See full target information NCOA3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 9:895190 PubMed35898549

2022

Chimeric RNA TNNI2-ACTA1-V1 Regulates Cell Proliferation by Regulating the Expression of NCOA3.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyu Liu,Jiaxin Li,Wanjun Hao,Xu Lin,Jiqiao Xia,Jiyuan Zhu,Shuo Yang,Xiuqin Yang

Molecular endocrinology (Baltimore, Md.) 19:644-56 PubMed15550471

2004

Overexpression of an N-terminally truncated isoform of the nuclear receptor coactivator amplified in breast cancer 1 leads to altered proliferation of mammary epithelial cells in transgenic mice.

Applications

Species

Mouse

Maddalena T Tilli,Ronald Reiter,Annabell S Oh,Ralf T Henke,Kevin McDonnell,G Ian Gallicano,Priscilla A Furth,Anna Tate Riegel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SRC3 antibody

Immunoprecipitation - Anti-SRC3 antibody (AB10310)

Western blot - Anti-SRC3 antibody (AB10310)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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