Anti-SRC3 antibody [AX15.3] - ChIP Grade

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (AB2782)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human pancreas tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2782 (1 : 20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting

• WB

Unknown

Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (AB2782)

Western blot of AIB1 on MCF-7 cell extract using ab2782. Western blot of AIB1 on MCF-7 cell extract using ab2782.

All lanes:

Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

Predicted band size: 155 kDa

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