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AB327216

Anti-SRCAP Antibody

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Rabbit Polyclonal SRCAP antibody. Suitable for IHC-P, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SRCAP.

View Alternative Names

KIAA0309, SRCAP, Chromatin remodeling protein SRCAP, Domino homolog 2, Snf2-related CBP activator

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRCAP Antibody (AB327216)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRCAP Antibody (AB327216)

Immunohistochemical staining of human Liver using ab327216 at 1 : 50–1 : 200 dilution, showing very weak nuclear positivity in hepatocytes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRCAP Antibody (AB327216)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRCAP Antibody (AB327216)

Immunohistochemical staining of human Cerebellum using ab327216 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in Purkinje cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRCAP Antibody (AB327216)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRCAP Antibody (AB327216)

Immunohistochemical staining of human Skin using ab327216 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in squamous epithelial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRCAP Antibody (AB327216)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRCAP Antibody (AB327216)

Immunohistochemical staining of human Testis using ab327216 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in cells in seminiferous ducts and leydig cells.

ChIP - Anti-SRCAP Antibody (AB327216)
  • ChIP

Supplier Data

ChIP - Anti-SRCAP Antibody (AB327216)

ChIP-Exo-Seq composite graph for ab327216 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ChIP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SRCAP.

Q6ZRS2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts both as a chromatin remodeler and transcription coregulator. Catalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes expression through chromatin remodeling (PubMed : 16634648, PubMed : 17617668). Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription (PubMed : 10347196, PubMed : 11522779, PubMed : 14500758, PubMed : 16024792).
See full target information SRCAP
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Product promise

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