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AB27469

Anti-SRD5A2 antibody

4

(1 Review)

|

(5 Publications)

Goat Polyclonal SRD5A2 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SRD5A2 aa 1-50.

View Alternative Names

3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, SR type 2, Steroid 5-alpha-reductase 2, Type II 5-alpha reductase, S5AR 2, SRD5A2

1 Images
Western blot - Anti-SRD5A2 antibody (AB27469)
  • WB

Unknown

Western blot - Anti-SRD5A2 antibody (AB27469)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-SRD5A2 antibody (ab27469) at 0.3 µg/mL

All lanes:

Human testes lysate (35ug protein in RIPA buffer)

Predicted band size: 28 kDa

Observed band size: 26 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SRD5A2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P31213

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
See full target information SRD5A2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell stem cell 27:876-889.e12 PubMed33232663

2020

Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan M Samuel,Homa Majd,Mikayla N Richter,Zaniar Ghazizadeh,Seyedeh Maryam Zekavat,Albertas Navickas,Jonathan T Ramirez,Hosseinali Asgharian,Camille R Simoneau,Luke R Bonser,Kyung Duk Koh,Miguel Garcia-Knight,Michel Tassetto,Sara Sunshine,Sina Farahvashi,Ali Kalantari,Wei Liu,Raul Andino,Hongyu Zhao,Pradeep Natarajan,David J Erle,Melanie Ott,Hani Goodarzi,Faranak Fattahi

Developmental neuroscience 37:533-45 PubMed26279160

2015

Prenatal Stress Alters Hippocampal Neuroglia and Increases Anxiety in Childhood.

Applications

Unspecified application

Species

Unspecified reactive species

Greer A Bennett,Hannah K Palliser,Julia C Shaw,David Walker,Jonathan J Hirst

Reproductive sciences (Thousand Oaks, Calif.) 20:1365-75 PubMed23585339

2013

Changes in neuroactive steroid concentrations after preterm delivery in the Guinea pig.

Applications

Unspecified application

Species

Unspecified reactive species

Meredith A Kelleher,Jonathan J Hirst,Hannah K Palliser

The Journal of endocrinology 208:301-9 PubMed21149437

2010

Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development.

Applications

Unspecified application

Species

Unspecified reactive species

Meredith A Kelleher,Hannah K Palliser,David W Walker,Jonathan J Hirst

Reproduction, fertility, and development 21:599-607 PubMed19383266

2009

Changes in human placental 5alpha-reductase isoenzyme expression with advancing gestation: effects of fetal sex and glucocorticoid exposure.

Applications

WB

Species

Human

Thi T Vu,Jonathan J Hirst,Michael Stark,Ian M R Wright,Hannah K Palliser,Nicolette Hodyl,Vicki L Clifton
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB124877

Anti-SRD5A2 antibody [EPR6281(B)]

primary-antibodies

srd5a2-antibody-epr6281b-ab124877

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(0 reviews)

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