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AB124877

Anti-SRD5A2 antibody [EPR6281(B)]

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(6 Publications)

Rabbit Recombinant Monoclonal SRD5A2 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

3-oxo-5-alpha-steroid 4-dehydrogenase 2, 5 alpha-SR2, SR type 2, Steroid 5-alpha-reductase 2, Type II 5-alpha reductase, S5AR 2, SRD5A2

2 Images
Western blot - Anti-SRD5A2 antibody [EPR6281(B)] (AB124877)
  • WB

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Western blot - Anti-SRD5A2 antibody [EPR6281(B)] (AB124877)

All lanes:

Western blot - Anti-SRD5A2 antibody [EPR6281(B)] (ab124877) at 1/1000 dilution

Lane 1:

LnCaP cell lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Lane 3:

PC3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 28 kDa

false

OI-RD Scanning - Anti-SRD5A2 antibody [EPR6281(B)] (AB124877)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SRD5A2 antibody [EPR6281(B)] (AB124877)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6281(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
See full target information SRD5A2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:183 PubMed38491517

2024

Dual deficiency of melatonin and dihydrotestosterone promotes stromal cell damage and mediates prostatitis via the cGAS-STING pathway in sleep-deprived mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Chen,Wenming Ma,Shaoyu Yue,Dongsheng Li,Lei Chen,Cheng Zhang,Yu Guan,Chun Li,Changqin Jiang,Guiyi Liao,Chaozhao Liang,Hui Wang,Sheng Tai

Stem cell research & therapy 14:219 PubMed37612726

2023

Cell-free fat extract restores hair loss: a novel therapeutic strategy for androgenetic alopecia.

Applications

Unspecified application

Species

Unspecified reactive species

Yizuo Cai,Zhuoxuan Jia,Yichen Zhang,Bijun Kang,Chingyu Chen,Wei Liu,Wei Li,Wenjie Zhang

Nutrition research and practice 16:419-434 PubMed35919286

2022

Ameliorative effect of Nakai on benign prostatic hyperplasia and .

Applications

Unspecified application

Species

Unspecified reactive species

Young-Jin Choi,Meiqi Fan,Yujiao Tang,Sangho Moon,Seung-Hyun Lee,Bokyung Lee,Sung Mun Bae,Sang Moo Lee,Eun-Kyung Kim

The Journal of steroid biochemistry and molecular biology 198:105527 PubMed31733346

2019

Role of human 3α-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C3) in the extrahepatic metabolism of the steroidal aromatase inactivator Formestane.

Applications

Unspecified application

Species

Unspecified reactive species

Runlan Wan,Xi Kong,Youzhe Yang,Siwen Tao,Youyou Chen,Alexander Tobias Teichmann,Frank Heinrich Wieland

Nutrients 11: PubMed31615010

2019

In Vivo Effects of Polymerized Anthocyanin from Grape Skin on Benign Prostatic Hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Jin Choi,Meiqi Fan,Yujiao Tang,Hyun Pil Yang,Ji-Young Hwang,Eun-Kyung Kim

Journal of cell science 125:3590-600 PubMed22595520

2012

Regulation of multiple target genes by miR-1 and miR-206 is pivotal for C2C12 myoblast differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Goljanek-Whysall,Helio Pais,Tina Rathjen,Dylan Sweetman,Tamas Dalmay,Andrea Münsterberg
View all publications
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Product promise

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