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AB269577

Anti-SREBP1 antibody [2A4] - BSA and Azide free

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Mouse Monoclonal SREBP1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
1 Images
Western blot - Anti-SREBP1 antibody [2A4] - BSA and Azide free (AB269577)
  • WB

Lab

Western blot - Anti-SREBP1 antibody [2A4] - BSA and Azide free (AB269577)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab3259 and ab181602 (rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

This image was produced using the same antibody clone but in a different formulation ab3259, PBS and sodium azide.

All lanes:

Western blot - Anti-SREBP1 antibody [2A4] (<a href='/en-us/products/primary-antibodies/srebp1-antibody-2a4-ab3259'>ab3259</a>) at 5 µg/mL

Lane 1:

HeLa whole cell lysate at 40 µg

Lane 2:

HAP1 whole cell lysate at 40 µg

Predicted band size: 122 kDa

Observed band size: 125 kDa,65 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A4

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Amino acids 301 - 407.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p>By Western blot, this antibody detects bands of 125 kDa (precursor SREBP1) and 60 - 70 kDa (cleaved SREBP1).</p><p>Based on in-house testing, Abcam recommends using a 5% milk block for this product.</p>" } } }
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Product details

ab269577 is the carrier-free version of ab3259.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sterol regulatory element-binding protein 1. Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane (PubMed : 32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis (By similarity).. Processed sterol regulatory element-binding protein 1. Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis (PubMed : 12177166, PubMed : 32322062, PubMed : 8402897). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed : 12177166, PubMed : 8402897). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation (PubMed : 12177166, PubMed : 32322062, PubMed : 8402897).. Isoform SREBP-1A. Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C (By similarity). Able to stimulate both lipogenic and cholesterogenic gene expression (PubMed : 12177166, PubMed : 32497488). Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver (By similarity). Required for innate immune response in macrophages by regulating lipid metabolism (By similarity).. Isoform SREBP-1C. Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A (By similarity). Primarily controls expression of lipogenic gene (PubMed : 12177166). Strongly activates global lipid synthesis in rapidly growing cells (By similarity).. Isoform SREBP-1aDelta. The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters.. Isoform SREBP-1cDelta. The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters.
See full target information SREBF1

Alternative Names

BHLHD1, SREBP1, SREBF1, Sterol regulatory element-binding protein 1, SREBP-1, Class D basic helix-loop-helix protein 1, Sterol regulatory element-binding transcription factor 1, bHLHd1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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