Anti-SREBP1 antibody [EPR27079-194]

• WB

Supplier Data

Western blot - Anti-SREBP1 antibody [EPR27079-194] (AB313881)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-2 : 180 seconds, Lane 3-4 : 15 seconds.

All lanes:

Western blot - Anti-SREBP1 antibody [EPR27079-194] (ab313881) at 1/1000 dilution

Lane 1:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Lane 2:

3T3-L1 differentiation for 6 days whole cell lysate at 40 µg

Lane 3:

Hela (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

Lane 4:

Hela transfected with siRNA specifically targeti SREBP1 whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 122 kDa

Observed band size: 125 kDa

false

• WB

Lab

Western blot - Anti-SREBP1 antibody [EPR27079-194] (AB313881)

Western blot : Anti-SREBF1/SREBP1 antibody [EPR27079-194] (ab313881) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab313881 was shown to bind specifically to SREBF1/SREBP1. A band was observed at 125 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in SREBF1/SREBP1 knockout cell line. To generate this image, wild-type and SREBF1/SREBP1 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-SREBP1 antibody [EPR27079-194] (ab313881) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

SREBF1/SREBP1 knockout HCT 116 cell lysate at 20 µg

Lane 3:

Wild-type A549 ab288558 cell lysate at 20 µg

Lane 4:

HT-29 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 125 kDa

false

• WB

Supplier Data

Western blot - Anti-SREBP1 antibody [EPR27079-194] (AB313881)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 180 seconds

All lanes:

Western blot - Anti-SREBP1 antibody [EPR27079-194] (ab313881) at 1/1000 dilution

All lanes:

Human adrenal gland tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 122 kDa

Observed band size: 125 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-SREBP1 antibody [EPR27079-194] (AB313881)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 7 seconds

All lanes:

Western blot - Anti-SREBP1 antibody [EPR27079-194] (ab313881) at 1/1000 dilution

All lanes:

Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 122 kDa

Observed band size: 125 kDa

false

Exposure time: 7s

• WB

Lab

Western blot - Anti-SREBP1 antibody [EPR27079-194] (AB313881)

Western blot : Recombinant Anti-SREBP1 antibody [EPR27079-194] ab313881 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 125 kDa in Wild-type HeLa cell lysates with no signal observed at this size in SREBF1 knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SREBP1 antibody [EPR27079-194] (ab313881) at 1/1000 dilution

Lane 1:

Wild-type HeLa at 20 µg

Lane 2:

Western blot - Human SREBF1 (SREBP1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-srebf1-srebp1-knockout-hela-cell-line-ab277151'>ab277151</a>) at 20 µg

Lane 3:

Wild-type HCT 116 ab288559 at 20 µg

Lane 4:

Western blot - Human SREBF1 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-srebf1-knockout-hct116-cell-line-ab289113'>ab289113</a>) at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

false

• WB

Supplier Data

Western blot - Anti-SREBP1 antibody [EPR27079-194] (AB313881)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1 : 70 seconds, Lane 2 : 26 seconds

All lanes:

Western blot - Anti-SREBP1 antibody [EPR27079-194] (ab313881) at 1/1000 dilution

Lane 1:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

Mouse liver tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 122 kDa

Observed band size: 125 kDa

false