AB28482

Anti-SREBP2 antibody

Name: Anti-SREBP2 antibody (ab28482) | Abcam
Brand: Abcam Limited
SKU: AB28482
Rating: 1 (1 reviews)

(1 Review)

(28 Publications)

Rabbit Polyclonal SREBP2 antibody. Suitable for ICC/IF and reacts with Rat, Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Rat Srebf2 aa 850-900.

View Alternative Names

Sterol regulatory element-binding protein 2, SREBP-2, Sterol regulatory element-binding transcription factor 2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB28482)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB28482)

Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB28482)

Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of rat L6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB28482)

Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of Rat PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1 : 100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Rat Srebf2 aa 850-900. The exact immunogen used to generate this antibody is proprietary information.

Q3T1I5

Specificity

ab28482 recognises Sterol regulatory element-binding protein 2 (SREBP 2)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sterol regulatory element-binding protein 2. Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis.. Processed sterol regulatory element-binding protein 2. Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates transcription of genes related to cholesterol synthesis pathway. Regulates hepatic lipogenesis.

See full target information Srebf2

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience research 204:22-33 PubMed38278219

2024

Relationships of brain cholesterol and cholesterol biosynthetic enzymes to Alzheimer's pathology and dementia in the CFAS population-derived neuropathology cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Hemant Mistry,Connor D Richardson,Adrian Higginbottom,Bridget Ashford,Saif U Ahamed,Zoe Moore,Fiona E Matthews,Carol Brayne,Julie E Simpson,Stephen B Wharton

Diabetologia 67:356-370 PubMed38032369

2023

RNA sequencing unravels novel L cell constituents and mechanisms of GLP-1 secretion in human gastric bypass-operated intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Michael G Miskelly,Andreas Lindqvist,Elena Piccinin,Alexander Hamilton,Elaine Cowan,Bent-Johnny Nergård,Rita Del Giudice,Mtakai Ngara,Luis R Cataldo,Dmytro Kryvokhyzha,Petr Volkov,Luke Engelking,Isabella Artner,Jens O Lagerstedt,Lena Eliasson,Emma Ahlqvist,Antonio Moschetta,Jan Hedenbro,Nils Wierup

International journal of molecular sciences 23: PubMed36555149

2022

Butyrate Lowers Cellular Cholesterol through HDAC Inhibition and Impaired SREBP-2 Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Bridgeman,Hon Chiu Woo,Philip Newsholme,Cyril Mamotte

Journal of dairy science 105:7773-7786 PubMed35940922

2022

Role of sortilin 1 (SORT1) on fatty acid-mediated cholesterol metabolism in primary calf hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Wang,Qianming Jiang,Juan J Loor,Changhong Gao,Mingmao Yang,Yan Tian,Wenwen Fan,Bingbing Zhang,Ming Li,Chuang Xu,Wei Yang

Oxidative medicine and cellular longevity 2022:7280312 PubMed35498136

2022

Screening of lncRNA-miRNA-mRNA Coexpression Regulatory Networks Involved in Acute Traumatic Coagulation Dysfunction Based on CTD, GeneCards, and PharmGKB Databases.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Luo,Yong Fu,Taifa Tan,Jun Hu,Fang Li,Zhanchen Liao,Jian Peng

International journal of molecular sciences 23: PubMed35328827

2022

Brain Cholesterol Biosynthetic Pathway Is Altered in a Preclinical Model of Fragile X Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Parente,Claudia Tonini,Valeria Buzzelli,Emilia Carbone,Viviana Trezza,Valentina Pallottini

Cancers 12: PubMed33352737

2020

Ouabain and Digoxin Activate the Proteasome and the Degradation of the ERα in Cells Modeling Primary and Metastatic Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Busonero,Stefano Leone,Fabrizio Bianchi,Elena Maspero,Marco Fiocchetti,Orazio Palumbo,Manuela Cipolletti,Stefania Bartoloni,Filippo Acconcia

Frontiers in veterinary science 7:560241 PubMed33195535

2020

Novel HI120 Affects Lipid Metabolism in C57BL/6 Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Sun,Yanqing Tang,Xufeng Hou,Hesong Wang,Liuying Huang,Junjie Wen,Hongxin Niu,Weisen Zeng,Yang Bai

Food & function 11:8369-8379 PubMed32935679

2020

L-Theanine affects intestinal mucosal immunity by regulating short-chain fatty acid metabolism under dietary fiber feeding.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xu,Ling Lin,An Liu,Tuo Zhang,Sheng Zhang,Yinhua Li,Jinhua Chen,Zhihua Gong,Zhonghua Liu,Wenjun Xiao

Physiological genomics 52:160-167 PubMed32036767

2020

Characterization of the promoter region of the bovine 3 gene: roles of SREBF2 and PPARG.

Applications

Unspecified application

Species

Unspecified reactive species

Dawei Wei,Sayed Haidar Abbas Raza,Jiupan Zhang,Xingping Wang,Rajwali Khan,Yun Ma,Guijie Zhang,Zhuoma Luoreng,Linsen Zan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SREBP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB28482)

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB28482)

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB28482)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (28)

Product promise