Anti-SREBP2 antibody ab30682 is a rabbit polyclonal antibody that is used in SREBP2 western blotting, IHC and immunofluorescence. Suitable for human and rat samples.
- Tried and trusted by researchers since 2006
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- Nature communications 14:80752023Enhanced SREBP2-driven cholesterol biosynthesis by PKCλ/ι deficiency in intestinal epithelial cells promotes aggressive serrated tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Muta,Juan F Linares,Anxo Martinez-Ordoñez,Angeles Duran,Tania Cid-Diaz,Hiroto Kinoshita,Xiao Zhang,Qixiu Han,Yuki Nakanishi,Naoko Nakanishi,Thekla Cordes,Gurpreet K Arora,Marc Ruiz-Martinez,Miguel Reina-Campos,Hiroaki Kasashima,Masakazu Yashiro,Kiyoshi Maeda,Ana Albaladejo-Gonzalez,Daniel Torres-Moreno,José García-Solano,Pablo Conesa-Zamora,Giorgio Inghirami,Christian M Metallo,Timothy F Osborne,Maria T Diaz-Meco,Jorge MoscatPubMed 38092754
- Nutrients 15:2023Virgin Camellia Seed Oil Improves Glycolipid Metabolism in the Kidney of High Fat-Fed Rats through AMPK-SREBP Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinhe Zhu,Guihui Li,Li Ma,Bolin Chen,Dawei Zhang,Jing Gao,Senwen Deng,Yongzhong ChenPubMed 38068746
- Cell death & disease 14:4742023Statins suppress cell-to-cell propagation of α-synuclein by lowering cholesterol.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoo-Ok Min,Hoang-Anh Ho,Wonjae Lee,Byung Chul Jung,Sung Jun Park,Seokjoong Kim,Seung-Jae LeePubMed 37500624
- PLoS neglected tropical diseases 17:e00114772023Single-cell sequencing analysis reveals development and differentiation trajectory of Schwann cells manipulated by M. leprae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Ma,Zihao Mi,Zhenzhen Wang,Lele Sun,Tingting Liu,Peidian Shi,Chuan Wang,Xiaotong Xue,Wenjie Chen,Zhe Wang,Yueqian Yu,Yuan Zhang,Fangfang Bao,Na Wang,Honglei Wang,Qianqian Xia,Hong Liu,Yonghu Sun,Furen ZhangPubMed 37478057
- Acta neuropathologica communications 11:1022023DHCR24 reverses Alzheimer's disease-related pathology and cognitive impairment via increasing hippocampal cholesterol levels in 5xFAD mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Bin Zhang,Yue Huang,Xiao-Rou Guo,Meng-Qi Zhang,Xiang-Shan Yuan,Heng-Bing ZuPubMed 37344916
- Journal of translational medicine 21:3622023NUCB2/Nesfatin-1 drives breast cancer metastasis through the up-regulation of cholesterol synthesis via the mTORC1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyi Ning,Caiying Liu,Kangtao Wang,Yubo Cai,Zhicheng Ning,Ming Li,Liang ZengPubMed 37277807
- Cell death & disease 14:2492023SCAP contributes to embryonic angiogenesis by negatively regulating KISS-1 expression in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuo Zheng,Yu Su,Li Wei,Yingcheng Yao,Yizhe Wang,Xiaoting Luo,Xing Wang,Xiong Z Ruan,Danyang Li,Yaxi ChenPubMed 37024487
- Frontiers in pharmacology 14:10161292023Quantitative proteomics reveals ameliorates hepatic steatosis by promoting PPARs/CPT1A/CPT2-mediated fatty acid β-oxidation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuanjie Liu,Ling Chang,Yihan Qian,Jiacheng Lin,Zhi Shang,Min Xu,Fang Wang,Xuehua Sun,Yun Jiang,Yueqiu Gao,Xiaoni KongPubMed 37033635
- Andrology 11:1203-12172023Extra-virgin olive oil ameliorates high-fat diet-induced seminal and testicular disorders by modulating the cholesterol pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbi Karenina Funes,María Virginia Avena,Jorge Ibañez,Layla Simón,Leonor Ituarte,Regina Colombo,Adrián Roldán,María Inés Conte,María Ángeles Monclus,Paola Boarelli,Miguel Walter Fornés,Tania Estefanía Saez LancellottiPubMed 36695747
- International journal of molecular sciences 24:2023CircRNA-PI4KB Induces Hepatic Lipid Deposition in Non-Alcoholic Fatty Liver Disease by Transporting miRNA-122 to Extra-Hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang-Hai Liu,Wei Jiang,Qingmin Zeng,Dongbo Wu,Hong Li,Lingyun Zhou,Lang Bai,Hong TangPubMed 36674813
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human SREBF2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q12772
Reactivity data
Select an application| WB | ICC/IF | IHC-Fr | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Rat | Tested | Expected | Expected |
| Chicken | Predicted | Predicted | Expected |
| Chinese hamster | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/200.00000 - 1/1000.00000 | Notes Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C. |
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes Block with 5% non-fat dry milk in TBS pH 7.4 for 1-2 hours at room temperature or over night at 4°C. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig, Xenopus laevis, Chinese hamster, Chicken | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig, Xenopus laevis, Chinese hamster, Chicken | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chicken, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig, Pig, Xenopus laevis, Chinese hamster | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Sterol regulatory element-binding protein 2Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane (PubMed:32322062). Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis (PubMed:32322062).Processed sterol regulatory element-binding protein 2Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis (PubMed:12177166, PubMed:32322062). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3') (PubMed:7903453, PubMed:12177166). Regulates transcription of genes related to cholesterol synthesis pathway (PubMed:12177166, PubMed:32322062).
Alternative names
Sterol regulatory element-binding protein 2, SREBP-2, Class D basic helix-loop-helix protein 2, Sterol regulatory element-binding transcription factor 2, bHLHd2, SREBF2, BHLHD2, SREBP2
Recommended products
Anti-SREBP2 antibody ab30682 is a rabbit polyclonal antibody that is used in SREBP2 western blotting, IHC and immunofluorescence. Suitable for human and rat samples.
- Tried and trusted by researchers since 2006
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human SREBF2 aa 450-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q12772
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
This image is courtesy of a customer review by Dongil Kim.Western blot - Anti-SREBP2 antibody (ab30682)
Blocking: 7% milk for 60 minutes at 20oC.
All lanes: Western blot - Anti-SREBP2 antibody (ab30682) at 1/1000 dilution
All lanes: Human retina whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 123 kDa
Observed band size: 126 kDa, 55 kDa, 60 kDa
Exposure time: 6min
Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (ab30682)
ICC/IF image of ab30682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30682, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Western blot - Anti-SREBP2 antibody (ab30682)
Observed bands 126 kDa, 55kDa (cleaved form)
All lanes: Western blot - Anti-SREBP2 antibody (ab30682) at 1/200 dilution
Lane 1: Human fibroblast cell lysate at 60 µg
Lane 2: Rat brown fat homogenate at 60 µg
Lane 3: Rat testis supernatant at 60 µg
Predicted band size: 123 kDa
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