Rabbit Polyclonal SRGAP1 antibody. Suitable for IP, WB and reacts with Human, Transfected cell lysate - Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SRGAP1.
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Publications
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- Nature communications 10:23502019Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGael Genet et. al.PubMed 31138815
- Molecular biology of the cell 30:69-812018ADP-ribosylation factor-like 4A interacts with Robo1 to promote cell migration by regulating Cdc42 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTsai-Shin Chiang,Ming-Chieh Lin,Meng-Chen Tsai,Chieh-Hsin Chen,Li-Ting Jang,Fang-Jen S LeePubMed 30427759
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SRGAP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z6B7
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Bat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Transfected cell lysate - Human | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat, Elephant | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat, Elephant | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
Alternative names
ARHGAP13, KIAA1304, SRGAP1, SLIT-ROBO Rho GTPase-activating protein 1, srGAP1, Rho GTPase-activating protein 13
Recommended products
Rabbit Polyclonal SRGAP1 antibody. Suitable for IP, WB and reacts with Human, Transfected cell lysate - Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SRGAP1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SRGAP1. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z6B7
- Purification technique
- Affinity purification Immunogen
- Specificity
Does not crossreact with human srGAP2 or human srGAP3
- Concentration
- Purification notes
ab76926 was affinity purified using an epitope specific to SRGAP1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-SRGAP1 antibody (ab76926)
All lanes: Western blot - Anti-SRGAP1 antibody (ab76926) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate. at 50 µg
Lane 2: HeLa whole cell lysate. at 15 µg
Lane 3: HeLa whole cell lysate. at 5 µg
Lane 4: 293T whole cell lysate. at 50 µg
Predicted band size: 124 kDa
Observed band size: 150 kDa, 75 kDa, 80 kDa
Immunoprecipitation - Anti-SRGAP1 antibody (ab76926)
ab76926 at 1µg/ml staining SRGAP1 in HeLa whole cell lysate immunoprecipitated using ab76926 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
All lanes: Immunoprecipitation - Anti-SRGAP1 antibody (ab76926)
Predicted band size: 124 kDa
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