Rabbit Recombinant Monoclonal SRGAP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--western-blot-img106981.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938)")
![Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--flow-cytometry-intracellular-img79638.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938)")
![Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--immunocytochemistry-immunofluorescence-img26756.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130774.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img39958.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Predicted | Not recommended | Predicted | Predicted | Predicted |
| Rat | Predicted | Not recommended | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
Alternative names
ARHGAP13, KIAA1304, SRGAP1, SLIT-ROBO Rho GTPase-activating protein 1, srGAP1, Rho GTPase-activating protein 13
Recommended products
Rabbit Recombinant Monoclonal SRGAP1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR8760(2)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--western-blot-img106981.jpg "Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)")
Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
All lanes: Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938) at 1/1000 dilution
Lane 1: Human fetal brain tissue lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: A549 cell lysate at 10 µg
Lane 4: Human fetal lung tissue lysate at 10 µg
Lane 5: Human fetal kidney tissue lysate at 10 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
![Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--flow-cytometry-intracellular-img79638.jpg "Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)")
Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
Intracellular flow cytometric analysis of permeabilized 293T cellslabeling SRGAP1 with ab171938 at 1/100 (red)or a rabbit IgG (negative) (green).
![Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--immunocytochemistry-immunofluorescence-img26756.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)")
Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
Immunofluorescent analysis of A549 cells labeling SRGAP1 with ab171938 at 1/100 (green). DAPI nuclear staining (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130774.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with ab171938 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-ab171938--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img39958.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with ab171938 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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