Rabbit Recombinant Monoclonal SRGAP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--western-blot-img106981.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)")
![Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--flow-cytometry-intracellular-img79638.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)")
![Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--immunocytochemistry-immunofluorescence-img26756.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130774.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img39958.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Predicted | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
Alternative names
ARHGAP13, KIAA1304, SRGAP1, SLIT-ROBO Rho GTPase-activating protein 1, srGAP1, Rho GTPase-activating protein 13
Recommended products
Rabbit Recombinant Monoclonal SRGAP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8760(2)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249658 is the carrier-free version of Anti-SRGAP1 antibody [EPR8760(2)] ab171938.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--western-blot-img106981.jpg "Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)")
Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
This data was developed using Anti-SRGAP1 antibody [EPR8760(2)] ab171938, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (Anti-SRGAP1 antibody [EPR8760(2)] ab171938) at 1/1000 dilution
Lane 1: Human fetal brain tissue lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: A549 cell lysate at 10 µg
Lane 4: Human fetal lung tissue lysate at 10 µg
Lane 5: Human fetal kidney tissue lysate at 10 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
![Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--flow-cytometry-intracellular-img79638.jpg "Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)")
Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
This data was developed using Anti-SRGAP1 antibody [EPR8760(2)] ab171938, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized 293T cells labeling SRGAP1 with Anti-SRGAP1 antibody [EPR8760(2)] ab171938 at 1/100 (red) or a rabbit IgG (negative) (green).
![Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--immunocytochemistry-immunofluorescence-img26756.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)")
Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
This data was developed using Anti-SRGAP1 antibody [EPR8760(2)] ab171938, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A549 cells labeling SRGAP1 with Anti-SRGAP1 antibody [EPR8760(2)] ab171938 at 1/100 (green). DAPI nuclear staining (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130774.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
This data was developed using Anti-SRGAP1 antibody [EPR8760(2)] ab171938, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with Anti-SRGAP1 antibody [EPR8760(2)] ab171938 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658), expandable thumbnail](https://content.abcam.com/products/images/srgap1-antibody-epr87602-bsa-and-azide-free-ab249658--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img39958.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (ab249658)
This data was developed using Anti-SRGAP1 antibody [EPR8760(2)] ab171938, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with Anti-SRGAP1 antibody [EPR8760(2)] ab171938 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com