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AB249658

Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal SRGAP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ARHGAP13, KIAA1304, SRGAP1, SLIT-ROBO Rho GTPase-activating protein 1, srGAP1, Rho GTPase-activating protein 13

5 Images
Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)

This data was developed using ab171938, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labeling SRGAP1 with ab171938 at 1/100 (red) or a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)

This data was developed using ab171938, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with ab171938 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)

This data was developed using ab171938, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of A549 cells labeling SRGAP1 with ab171938 at 1/100 (green). DAPI nuclear staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)

This data was developed using ab171938, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with ab171938 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)
  • WB

Supplier Data

Western blot - Anti-SRGAP1 antibody [EPR8760(2)] - BSA and Azide free (AB249658)

This data was developed using ab171938, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (<a href='/en-us/products/primary-antibodies/srgap1-antibody-epr87602-ab171938'>ab171938</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

Human fetal lung tissue lysate at 10 µg

Lane 5:

Human fetal kidney tissue lysate at 10 µg

Predicted band size: 124 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8760(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249658 is the carrier-free version of ab171938.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
See full target information SRGAP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com