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AB121977

Anti-srGAP2 antibody

4

(2 Reviews)

|

(4 Publications)

Rabbit Polyclonal srGAP2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, African green monkey samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SRGAP2 aa 950 to C-terminus.

View Alternative Names

ARHGAP34, FNBP2, KIAA0456, SRGAP2A, SRGAP2, SLIT-ROBO Rho GTPase-activating protein 2, srGAP2, Formin-binding protein 2, Rho GTPase-activating protein 34

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunohistochemical analysis of human testis tissue labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in Leydig cells and seminiferous duct cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunohistochemical analysis of human kidney tissue labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in distal tube cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (AB121977)

Immunohistochemical analysis of human bone marrow labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in hematopoietic cells.

Immunocytochemistry/ Immunofluorescence - Anti-srGAP2 antibody (AB121977)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-srGAP2 antibody (AB121977)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-srGAP2 antibody (AB121977)
  • WB

Collaborator

Western blot - Anti-srGAP2 antibody (AB121977)

Blocking : 5% MIlk

All lanes:

Western blot - Anti-srGAP2 antibody (ab121977) at 1/100 dilution

Lane 1:

COS7 Whole cell lysate at 30 µg

Lane 2:

HEK293H Whole Cell Lysate at 30 µg

Lane 3:

HEK293H Over expressing human srGAP1 at 30 µg

Lane 4:

HEK293H Over expressing human srGAP2 at 30 µg

Lane 5:

HEK293H Over expressing human srGAP3 at 30 µg

Lane 6:

HeLa cells over expressing mouse srGAP2 at 30 µg

Lane 7:

Mouse E14 hindbrain lysate at 30 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution

Predicted band size: 121 kDa

Observed band size: 170 kDa,200 kDa

true

Exposure time: 5min

This image is courtesy of Alain Chedotal (Institut Vision, France)

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, African green monkey

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SRGAP2 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75044

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/20", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab121977 is mono-specific.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Postsynaptic RAC1 GTPase activating protein (GAP) that plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PubMed : 20810653, PubMed : 27373832, PubMed : 28333212). Regulates excitatory and inhibitory synapse maturation and density in cortical pyramidal neurons (PubMed : 22559944, PubMed : 27373832). SRGAP2/SRGAP2A limits excitatory and inhibitory synapse density through its RAC1-specific GTPase activating activity, while it promotes maturation of both excitatory and inhibitory synapses through its ability to bind to the postsynaptic scaffolding protein HOMER1 at excitatory synapses, and the postsynaptic protein GPHN at inhibitory synapses (By similarity). Mechanistically, acts by binding and deforming membranes, thereby regulating actin dynamics to regulate cell migration and differentiation (PubMed : 27373832). Promotes cell repulsion and contact inhibition of locomotion : localizes to protrusions with curved edges and controls the duration of RAC1 activity in contact protrusions (By similarity). In non-neuronal cells, may also play a role in cell migration by regulating the formation of lamellipodia and filopodia (PubMed : 20810653, PubMed : 21148482).
See full target information SRGAP2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Genes 16: PubMed40282329

2025

Techniques for Validating CRISPR Changes Using RNA-Sequencing Data.

Applications

Unspecified application

Species

Unspecified reactive species

Susan K Rathe,Tracy A Marko,Elizabeth N Edwards,Paige Hazelton Ridder,Jyotika Varshney,Kyle B Williams,James E Johnson,Branden S Moriarity,David A Largaespada

BMC nephrology 23:151 PubMed35436879

2022

Impact of klotho on the expression of SRGAP2a in podocytes in diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Donghua Jin,Miao Jia,Yuxian Xie,Lihua Lin,Hong Qiu,Guoyuan Lu

Diabetes 67:717-730 PubMed29242313

2017

Dissection of Glomerular Transcriptional Profile in Patients With Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Pan,Song Jiang,Qing Hou,Dandan Qiu,Jingsong Shi,Ling Wang,Zhaohong Chen,Mingchao Zhang,Aiping Duan,Weisong Qin,Ke Zen,Zhihong Liu

Scientific reports 6:39059 PubMed27966608

2016

Slit-Robo GTPase-Activating Protein 2 as a metastasis suppressor in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tracy A Marko,Ghaidan A Shamsan,Elizabeth N Edwards,Paige E Hazelton,Susan K Rathe,Ingrid Cornax,Paula R Overn,Jyotika Varshney,Brandon J Diessner,Branden S Moriarity,M Gerard O'Sullivan,David J Odde,David A Largaespada
View all publications
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Product promise

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