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AB124958

Anti-srGAP2 antibody [EP2506(2)]

3

(2 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal srGAP2 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 3 publications.

View Alternative Names

ARHGAP34, FNBP2, KIAA0456, SRGAP2A, SRGAP2, SLIT-ROBO Rho GTPase-activating protein 2, srGAP2, Formin-binding protein 2, Rho GTPase-activating protein 34

2 Images
Western blot - Anti-srGAP2 antibody [EP2506(2)] (AB124958)
  • WB

Supplier Data

Western blot - Anti-srGAP2 antibody [EP2506(2)] (AB124958)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-srGAP2 antibody [EP2506(2)] (ab124958) at 1/1000 dilution

All lanes:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa

false

Western blot - Anti-srGAP2 antibody [EP2506(2)] (AB124958)
  • WB

Supplier Data

Western blot - Anti-srGAP2 antibody [EP2506(2)] (AB124958)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-srGAP2 antibody [EP2506(2)] (ab124958) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa

false

  • Carrier free

    Anti-srGAP2 antibody [EP2506(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2506(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

This product has switched from a hybridoma to recombinant production method on 7th September 2023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Postsynaptic RAC1 GTPase activating protein (GAP) that plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PubMed : 20810653, PubMed : 27373832, PubMed : 28333212). Regulates excitatory and inhibitory synapse maturation and density in cortical pyramidal neurons (PubMed : 22559944, PubMed : 27373832). SRGAP2/SRGAP2A limits excitatory and inhibitory synapse density through its RAC1-specific GTPase activating activity, while it promotes maturation of both excitatory and inhibitory synapses through its ability to bind to the postsynaptic scaffolding protein HOMER1 at excitatory synapses, and the postsynaptic protein GPHN at inhibitory synapses (By similarity). Mechanistically, acts by binding and deforming membranes, thereby regulating actin dynamics to regulate cell migration and differentiation (PubMed : 27373832). Promotes cell repulsion and contact inhibition of locomotion : localizes to protrusions with curved edges and controls the duration of RAC1 activity in contact protrusions (By similarity). In non-neuronal cells, may also play a role in cell migration by regulating the formation of lamellipodia and filopodia (PubMed : 20810653, PubMed : 21148482).
See full target information SRGAP2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neuron 112:3602-3617.e9 PubMed39406239

2024

Synaptic neoteny of human cortical neurons requires species-specific balancing of SRGAP2-SYNGAP1 cross-inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Baptiste Libé-Philippot,Ryohei Iwata,Aleksandra J Recupero,Keimpe Wierda,Sergio Bernal Garcia,Luke Hammond,Anja van Benthem,Ridha Limame,Martyna Ditkowska,Sofie Beckers,Vaiva Gaspariunaite,Eugénie Peze-Heidsieck,Daan Remans,Cécile Charrier,Tom Theys,Franck Polleux,Pierre Vanderhaeghen

Cell reports 40:111358 PubMed36130489

2022

A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chandrani Mondal,Majo J Gacha-Garay,Kathryn A Larkin,Rebecca C Adikes,Julie S Di Martino,Chen-Chi Chien,Madison Fraser,Ireti Eni-Aganga,Esperanza Agullo-Pascual,Katarzyna Cialowicz,Umut Ozbek,Alexandra Naba,Angelo Gaitas,Tian-Ming Fu,Srigokul Upadhyayula,Eric Betzig,David Q Matus,Benjamin L Martin,Jose Javier Bravo-Cordero

Molecular biology and evolution 34:1463-1478 PubMed28333212

2017

Structural History of Human SRGAP2 Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Sporny,Julia Guez-Haddad,Annett Kreusch,Sivan Shakartzi,Avi Neznansky,Alice Cross,Michail N Isupov,Britta Qualmann,Michael M Kessels,Yarden Opatowsky
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com