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Rabbit Recombinant Monoclonal SRP19 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-SRP19 antibody [EPR8378] (AB131239), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP19 antibody [EPR8378] (AB131239), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SRP19 antibody [EPR8378] (AB131239), expandable thumbnail
  • OI-RD Scanning - Anti-SRP19 antibody [EPR8378] (AB131239), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Expected
Not recommended
Tested
Tested
Mouse
Predicted
Expected
Not recommended
Expected
Predicted
Rat
Predicted
Expected
Not recommended
Expected
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) (By similarity). Binds directly to 7SL RNA (By similarity). Mediates binding of SRP54 to the SRP complex (By similarity).

Alternative names

Signal recognition particle 19 kDa protein, SRP19

Recommended products

Rabbit Recombinant Monoclonal SRP19 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8378
Purification technique
Affinity purification Protein A
Dissociation constant
4.1 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-SRP19 antibody [EPR8378] (ab131239), expandable thumbnail

    Western blot - Anti-SRP19 antibody [EPR8378] (ab131239)

    All lanes: Western blot - Anti-SRP19 antibody [EPR8378] (ab131239) at 1/10000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: Daudi cell lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP19 antibody [EPR8378] (ab131239), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP19 antibody [EPR8378] (ab131239)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labelling SRP19 with ab131239 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SRP19 antibody [EPR8378] (ab131239), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SRP19 antibody [EPR8378] (ab131239)

    Immunofluorescence analysis of HepG2 cells labelling SRP19 with ab131239 at 1/100 dilution.

  • OI-RD Scanning - Anti-SRP19 antibody [EPR8378] (ab131239), expandable thumbnail

    OI-RD Scanning - Anti-SRP19 antibody [EPR8378] (ab131239)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com