Rabbit Recombinant Monoclonal SRP54 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-SRP54 antibody [EPR10851(B)] (AB154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--western-blot-img60711.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SRP54 antibody [EPR10851(B)] (AB154796)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91839.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112404.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130226.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73842.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)")
Publications
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- Molecular neurobiology 56:4051-40602018Intragenic Transcriptional cis-Antagonism Across SLC6A3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhao,Jinlong Yu,Juan Zhao,Xiaowu Chen,Nian Xiong,Tao Wang,Hong Qing,Zhicheng LinPubMed 30259411
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
| Mouse | Predicted | Predicted | Not recommended |
| Rat | Predicted | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) (PubMed:34020957). As part of the SRP complex, associates with the SRP receptor (SR) component SRPRA to target secretory proteins to the endoplasmic reticulum membrane (PubMed:34020957). Binds to the signal sequence of presecretory proteins when they emerge from the ribosomes (PubMed:34020957). Displays basal GTPase activity, and stimulates reciprocal GTPase activation of the SR subunit SRPRA (PubMed:28972538, PubMed:34020957). Forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SR subunit SRPRA (PubMed:34020957). SR compaction and GTPase mediated rearrangement of SR drive SRP-mediated cotranslational protein translocation into the ER (PubMed:34020957). Requires the presence of SRP9/SRP14 and/or SRP19 to stably interact with RNA (By similarity). Plays a role in proliferation and differentiation of granulocytic cells, neutrophils migration capacity and exocrine pancreas development (PubMed:28972538, PubMed:29914977).
Alternative names
Signal recognition particle subunit SRP54, Signal recognition particle 54 kDa protein, SRP54
Recommended products
Rabbit Recombinant Monoclonal SRP54 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR10851(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--western-blot-img60711.jpg "Western blot - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
Western blot - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
All lanes: Western blot - Anti-SRP54 antibody [EPR10851(B)] (ab154796) at 1/1000 dilution
Lane 1: Human placenta lysate at 10 µg
Lane 2: fetal brain lysate at 10 µg
Lane 3: 293T lysate at 10 µg
Lane 4: Jurkat lysate at 10 µg
Lane 5: HeLa lysate at 10 µg
Predicted band size: 56 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91839.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SRP54 with ab154796 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112404.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SRP54 with ab154796 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130226.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154796 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73842.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab154796 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133186.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154796 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134407.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)
Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154796 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133186.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796), expandable thumbnail](https://content.abcam.com/products/images/srp54-antibody-epr10851b-ab154796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134407.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (ab154796)")
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