AB154796

Anti-SRP54 antibody [EPR10851(B)]

RabMAb
Recombinant
What is this?

3

(2 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal SRP54 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Signal recognition particle subunit SRP54, Signal recognition particle 54 kDa protein, SRP54

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154796 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154796 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154796 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab154796 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SRP54 with ab154796 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SRP54 with ab154796 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

WB

Unknown

Western blot - Anti-SRP54 antibody [EPR10851(B)] (AB154796)

All lanes:

Western blot - Anti-SRP54 antibody [EPR10851(B)] (ab154796) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

fetal brain lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

Jurkat lysate at 10 µg

Lane 5:

HeLa lysate at 10 µg

Predicted band size: 56 kDa

false

Carrier free

Anti-SRP54 antibody [EPR10851(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10851(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER) (PubMed : 34020957). As part of the SRP complex, associates with the SRP receptor (SR) component SRPRA to target secretory proteins to the endoplasmic reticulum membrane (PubMed : 34020957). Binds to the signal sequence of presecretory proteins when they emerge from the ribosomes (PubMed : 34020957). Displays basal GTPase activity, and stimulates reciprocal GTPase activation of the SR subunit SRPRA (PubMed : 28972538, PubMed : 34020957). Forms a guanosine 5'-triphosphate (GTP)-dependent complex with the SR subunit SRPRA (PubMed : 34020957). SR compaction and GTPase mediated rearrangement of SR drive SRP-mediated cotranslational protein translocation into the ER (PubMed : 34020957). Requires the presence of SRP9/SRP14 and/or SRP19 to stably interact with RNA (By similarity). Plays a role in proliferation and differentiation of granulocytic cells, neutrophils migration capacity and exocrine pancreas development (PubMed : 28972538, PubMed : 29914977).

See full target information SRP54

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012766 PubMed39621795

2024

Flaviviruses induce ER-specific remodelling of protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ho Him Wong,Dorian Richard Kenneth Crudgington,Lewis Siu,Sumana Sanyal

Communications biology 4:1370 PubMed34876695

2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek Lee

Molecular neurobiology 56:4051-4060 PubMed30259411

2018

Intragenic Transcriptional cis-Antagonism Across SLC6A3.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhao,Jinlong Yu,Juan Zhao,Xiaowu Chen,Nian Xiong,Tao Wang,Hong Qing,Zhicheng Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com