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Rabbit Recombinant Monoclonal SRPK2 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113), expandable thumbnail
  • Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Expected
Tested
Mouse
Expected
Tested
Expected
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing (PubMed:9472028, PubMed:18559500, PubMed:21056976). Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression (PubMed:19592491). This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression (PubMed:21205200). Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation (PubMed:18559500). Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28 (PubMed:18425142). Probably by phosphorylating DDX23, leads to the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (PubMed:28076779). Can mediate hepatitis B virus (HBV) core protein phosphorylation (PubMed:12134018). Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles (PubMed:16122776).

Alternative names

SRSF protein kinase 2, SFRS protein kinase 2, Serine/arginine-rich protein-specific kinase 2, SR-protein-specific kinase 2, SRPK2

Recommended products

Rabbit Recombinant Monoclonal SRPK2 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication.

Alternative names

SRSF protein kinase 2, SFRS protein kinase 2, Serine/arginine-rich protein-specific kinase 2, SR-protein-specific kinase 2, SRPK2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR16366

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab251113 is the carrier-free version of Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113), expandable thumbnail

    Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113)

    This data was developed using Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 78 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113)

    This data was developed using Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Neuro-2a cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling SRPK2 with Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238 at 1/250 dilution (5.4 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
    Negative controls: anti-SRPK2 at 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113)

    This data was developed using Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling SRPK2 with Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113)

    This data was developed using Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SRPK2 with Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113), expandable thumbnail

    Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113)

    This data was developed using Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238) at 1/1000 dilution

    All lanes: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 78 kDa

  • Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113), expandable thumbnail

    Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (ab251113)

    This data was developed using Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (Anti-SRPK2 antibody [EPR16366] - N-terminal ab192238) at 1/2000 dilution

    All lanes: Human fetal brain lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 78 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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