Anti-SRPK2 antibody [EPR16366] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113)

This data was developed using ab192238, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113)

This data was developed using ab192238, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset : negative control).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113)

This data was developed using ab192238, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of Neuro-2a cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling SRPK2 with ab192238 at 1/250 dilution (5.4 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls : anti-SRPK2 at 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/500 dilution.

• WB

Supplier Data

Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113)

This data was developed using ab192238, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (<a href='/en-us/products/primary-antibodies/srpk2-antibody-epr16366-n-terminal-ab192238'>ab192238</a>) at 1/1000 dilution

All lanes:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 78 kDa

false

• WB

Supplier Data

Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113)

This data was developed using ab192238, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (<a href='/en-us/products/primary-antibodies/srpk2-antibody-epr16366-n-terminal-ab192238'>ab192238</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 78 kDa

false

• WB

Supplier Data

Western blot - Anti-SRPK2 antibody [EPR16366] - BSA and Azide free (AB251113)

This data was developed using ab192238, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (<a href='/en-us/products/primary-antibodies/srpk2-antibody-epr16366-n-terminal-ab192238'>ab192238</a>) at 1/2000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 78 kDa

false