Rabbit Recombinant Monoclonal SRPK2 antibody. N-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.
Images
![Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (AB192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--western-blot-img124135.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (AB192238)")
![Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - N-terminal (AB192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--immunocytochemistry-immunofluorescence-img13993.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - N-terminal (AB192238)")
![Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (AB192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--western-blot-img85266.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (AB192238)")
Publications
Filter by
- Frontiers in integrative neuroscience 15:7423772022SRPK2 Expression and Beta-Amyloid Accumulation Are Associated With BV2 Microglia Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqi Tian,Wenfang Zeng,Cuihuan Yan,Qiang Li,Nan Li,Lin Ruan,Jie Li,Xiaoguang Yao,Si LiPubMed 35153686
- Frontiers in aging neuroscience 11:3652020Loss of miR-369 Promotes Tau Phosphorylation by Targeting the Fyn and Serine/Threonine-Protein Kinase 2 Signaling Pathways in Alzheimer's Disease Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoguang Yao,Xiaohui Xian,Mingxing Fang,Shujuan Fan,Wenbin LiPubMed 32082134
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing (PubMed:9472028, PubMed:18559500, PubMed:21056976). Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression (PubMed:19592491). This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression (PubMed:21205200). Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation (PubMed:18559500). Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28 (PubMed:18425142). Probably by phosphorylating DDX23, leads to the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (PubMed:28076779). Can mediate hepatitis B virus (HBV) core protein phosphorylation (PubMed:12134018). Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles (PubMed:16122776).
Alternative names
SRSF protein kinase 2, SFRS protein kinase 2, Serine/arginine-rich protein-specific kinase 2, SR-protein-specific kinase 2, SRPK2
Recommended products
Rabbit Recombinant Monoclonal SRPK2 antibody. N-terminal. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR16366
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--western-blot-img124135.jpg "Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)")
Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)
All lanes: Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 78 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--immunocytochemistry-immunofluorescence-img13993.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)")
Immunocytochemistry/ Immunofluorescence - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)
Immunofluorescent analysis of Neuro-2a cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling SRPK2 with ab192238 at 1/250 dilution (5.4 μg/mL) followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Negative controls: anti-SRPK2 at 1/250 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) at 1/500 dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138626.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)
Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137486.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SRPK2 with ab192238 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--western-blot-img85266.jpg "Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)")
Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)
All lanes: Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/1000 dilution
All lanes: Human fetal kidney tissue lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 78 kDa
![Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--western-blot-img155363.jpg "Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)")
Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)
All lanes: Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238) at 1/2000 dilution
All lanes: Human fetal brain lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 78 kDa
![Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238), expandable thumbnail](https://content.abcam.com/products/images/srpk2-antibody-epr16366-n-terminal-ab192238--western-blot-img155363.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SRPK2 antibody [EPR16366] - N-terminal (ab192238)")
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