AB254935

Anti-SRSF10 antibody

Name: Anti-SRSF10 antibody (ab254935) | Abcam
Brand: Abcam Limited
SKU: AB254935
Price: 655 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal SRSF10 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SRSF10 aa 150-250.

View Alternative Names

FUSIP1, FUSIP2, SFRS13A, TASR, SRSF10, Serine/arginine-rich splicing factor 10, 40 kDa SR-repressor protein, FUS-interacting serine-arginine-rich protein 1, Splicing factor SRp38, TLS-associated protein with Ser-Arg repeats, TLS-associated serine-arginine protein, SRrp40, TLS-associated protein with SR repeats, TLS-associated SR protein

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

Formalin-fixed, paraffin-embedded human rectum tissue stained for SRSF10 with ab254935 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

Formalin-fixed, paraffin-embedded human testis tissue stained for SRSF10 with ab254935 at a 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for SRSF10 with ab254935 at a 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SRSF10 antibody (AB254935)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SRSF10 antibody (AB254935)

PFA fixed, Triton X-100 permeabilized HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling SRSF10 using ab254935 at 4μg/ml (green) in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for SRSF10 with ab254935 at a 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SRSF10 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O75494

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing (PubMed : 11684676, PubMed : 12419250, PubMed : 14765198). Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70 (PubMed : 14765198). Required for splicing repression in M-phase cells and after heat shock (PubMed : 14765198). Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing (PubMed : 26876937). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed : 26876937). May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator (PubMed : 12419250).

See full target information SRSF10

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40439883

2025

SRSF10 regulates oligodendrocyte differentiation during mouse central nervous system development by modulating pre-mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Yawei Mu,Zixuan Wei,Menghan Sun,Junjie Li,Yi Jiang,Hanyang Jiang,Ankangzhi Ma,Cuiqing Zhu,Xianhua Chen

Journal of translational medicine 21:879 PubMed38049848

2023

Altered splicing machinery in lung carcinoids unveils NOVA1, PRPF8 and SRSF10 as novel candidates to understand tumor biology and expand biomarker discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo Blázquez-Encinas,Víctor García-Vioque,Teresa Caro-Cuenca,María Trinidad Moreno-Montilla,Federica Mangili,Emilia Alors-Pérez,Sebastian Ventura,Aura D Herrera-Martínez,Paula Moreno-Casado,Marco A Calzado,Ángel Salvatierra,María A Gálvez-Moreno,Lynnette Fernandez-Cuesta,Matthieu Foll,Raúl M Luque,Nicolas Alcala,Sergio Pedraza-Arevalo,Alejandro Ibáñez-Costa,Justo P Castaño

PLoS genetics 19:e1010797 PubMed37307272

2023

Retrotransposon LINE-1 bodies in the cytoplasm of piRNA-deficient mouse spermatocytes: Ribonucleoproteins overcoming the integrated stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara De Luca,Anuj Gupta,Alex Bortvin

iScience 26:107042 PubMed37360696

2023

SRSF10 regulates proliferation of neural progenitor cells and affects neurogenesis in developing mouse neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Li,Hanyang Jiang,Yawei Mu,Zixuan Wei,Ankangzhi Ma,Menghan Sun,Jingjing Zhao,Cuiqing Zhu,Xianhua Chen

eLife 11: PubMed36355419

2022

SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Liu,Xukun Lu,Zheng-Hui Zhao,Ruibao Su,Qian-Nan Li Li,Yue Xue,Zheng Gao,Si-Min Sun Sun,Wen-Long Lei,Lei Li,Geng An,Hanyan Liu,Zhiming Han,Ying-Chun Ouyang,Yi Hou,Zhen-Bo Wang,Qing-Yuan Sun,Jianqiao Liu

Cell death & disease 12:764 PubMed34344861

2021

Alternatively spliced ANLN isoforms synergistically contribute to the progression of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Erliang Guo,Xionghui Mao,Xueying Wang,Lunhua Guo,Changming An,Cong Zhang,Kaibin Song,Guohui Wang,Chunbin Duan,Xiwei Zhang,Xianguang Yang,Zhennan Yuan,Ji Sun,Xiaomei Li,Weiwei Yang,Hongxue Meng,Susheng Miao

Cancer biology & therapy 22:311-323 PubMed33879018

2021

LINC00668 cooperated with HuR dependent upregulation of PKN2 to facilitate gastric cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jutang Li,Wei Dong,Qixia Jiang,Fenglian Zhang,Hui Dong

The journal of gene medicine 22:e3250 PubMed32639657

2020

Long non-coding RNA AGAP2-AS1 accelerates cell proliferation, migration, invasion and the EMT process in colorectal cancer via regulating the miR-4,668-3p/SRSF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hesheng Li,Song Guo,Mingkai Zhang,Lin Li,Feng Wang,Bingtan Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SRSF10 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

Immunocytochemistry/ Immunofluorescence - Anti-SRSF10 antibody (AB254935)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF10 antibody (AB254935)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (8)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

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