Rabbit Polyclonal SRSF3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SRSF3.
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- Cell death & disease 14:5112023SRSF3/AMOTL1 splicing axis promotes the tumorigenesis of nasopharyngeal carcinoma through regulating the nucleus translocation of YAP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Chen Xu,Jia-Xin Jiang,Ya-Qing Zhou,Shuai He,Yang Liu,Yi-Qi Li,Pan-Pan Wei,Jin-Xin Bei,Jian Sun,Chun-Ling LuoPubMed 37558679
- Cell 169:1051-1065.e182017The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeniz Simsek,Gerald C Tiu,Ryan A Flynn,Gun W Byeon,Kathrin Leppek,Adele F Xu,Howard Y Chang,Maria BarnaPubMed 28575669
- International journal of biological sciences 11:1248-562015Adapted Resistance to the Knockdown Effect of shRNA-Derived Srsf3 siRNAs in Mouse Littermates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasahiko Ajiro et. al.PubMed 26435690
- BioMedicine 5:102015P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Ju Lin et. al.PubMed 26048696
- Hepatology (Baltimore, Md.) 61:171-832014Deletion of serine/arginine-rich splicing factor 3 in hepatocytes predisposes to hepatocellular carcinoma in mice.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SRSF3. The exact immunogen used to generate this antibody is proprietary information. Database link P84103
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed:17036044, PubMed:26876937, PubMed:32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed:26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed:11336712, PubMed:18364396, PubMed:28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244).
Alternative names
SFRS3, SRP20, SRSF3, Serine/arginine-rich splicing factor 3, Pre-mRNA-splicing factor SRP20
Recommended products
Rabbit Polyclonal SRSF3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SRSF3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SRSF3. The exact immunogen used to generate this antibody is proprietary information. Database link P84103
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
This image is courtesy of Suypriya SenWestern blot - Anti-SRSF3 antibody (ab125124)
All lanes: Western blot - Anti-SRSF3 antibody (ab125124) at 1/2000 dilution
Lanes 1 - 2: SRSF3 knock-out Mouse hepatocyte cell lysate at 25 µg
Lanes 3 - 4: Wild type Mouse hepatocyte cell lysate at 25 µg
SecondaryAll lanes: Anti-rabbit IgG polyclonal HRP conjugate at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 19 kDa
Exposure time: 5s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (ab125124)
ab125124, at 5ug/ml, staining SRSF3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-SRSF3 antibody (ab125124)
All lanes: Western blot - Anti-SRSF3 antibody (ab125124) at 1/500 dilution
Lane 1: HeLa cell extract
Lane 2: Jurkat cell extract
Lane 3: Jurkat cell extract with synthesized peptide
Predicted band size: 19 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (ab125124)
ab125124 at 1/100 dilution staining SRSF3 in MCF7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.
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