AB125124

Anti-SRSF3 antibody

Name: Anti-SRSF3 antibody (ab125124) | Abcam
Brand: Abcam Limited
SKU: AB125124
Rating: 5 (3 reviews)

(3 Reviews)

(5 Publications)

Rabbit Polyclonal SRSF3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SRSF3.

View Alternative Names

SFRS3, SRP20, SRSF3, Serine/arginine-rich splicing factor 3, Pre-mRNA-splicing factor SRP20

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (AB125124)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (AB125124)

ab125124, at 5ug/ml, staining SRSF3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB125124)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB125124)

ab125124 at 1/100 dilution staining SRSF3 in MCF7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

Unknown

Western blot - Anti-SRSF3 antibody (AB125124)

All lanes:

Western blot - Anti-SRSF3 antibody (ab125124) at 1/500 dilution

Lane 1:

HeLa cell extract

Lane 2:

Jurkat cell extract

Lane 3:

Jurkat cell extract with synthesized peptide

Predicted band size: 19 kDa

false

AbReview35828****

Western blot - Anti-SRSF3 antibody (AB125124)

All lanes:

Western blot - Anti-SRSF3 antibody (ab125124) at 1/2000 dilution

Lanes 1 - 2:

SRSF3 knock-out Mouse hepatocyte cell lysate at 25 µg

Lanes 3 - 4:

Wild type Mouse hepatocyte cell lysate at 25 µg

Secondary

All lanes:

Anti-rabbit IgG polyclonal HRP conjugate at 1/5000 dilution

Predicted band size: 19 kDa

false

Exposure time: 5s

This image is courtesy of Suypriya Sen

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SRSF3. The exact immunogen used to generate this antibody is proprietary information.

P84103

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed : 17036044, PubMed : 26876937, PubMed : 32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed : 26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed : 11336712, PubMed : 18364396, PubMed : 28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed : 11336712, PubMed : 18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1 : interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244).

See full target information SRSF3

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:511 PubMed37558679

2023

SRSF3/AMOTL1 splicing axis promotes the tumorigenesis of nasopharyngeal carcinoma through regulating the nucleus translocation of YAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Chen Xu,Jia-Xin Jiang,Ya-Qing Zhou,Shuai He,Yang Liu,Yi-Qi Li,Pan-Pan Wei,Jin-Xin Bei,Jian Sun,Chun-Ling Luo

Cell 169:1051-1065.e18 PubMed28575669

2017

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Simsek,Gerald C Tiu,Ryan A Flynn,Gun W Byeon,Kathrin Leppek,Adele F Xu,Howard Y Chang,Maria Barna

International journal of biological sciences 11:1248-56 PubMed26435690

2015

Adapted Resistance to the Knockdown Effect of shRNA-Derived Srsf3 siRNAs in Mouse Littermates.

Applications

Unspecified application

Species

Unspecified reactive species

Masahiko Ajiro,Rong Jia,Rui-Hong Wang,Chu-Xia Deng,Zhi-Ming Zheng

BioMedicine 5:10 PubMed26048696

2015

P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Ju Lin,Tsung-Jung Ho,Ting-Hsu Lin,Wei-Yi Hsu,Shao-Mei Huang,Chiu-Chu Liao,Chih-Ho Lai,Xiang Liu,Hsinyi Tsang,Chien-Chen Lai,Fuu-Jen Tsai

Hepatology (Baltimore, Md.) 61:171-83 PubMed25132062

2014

Deletion of serine/arginine-rich splicing factor 3 in hepatocytes predisposes to hepatocellular carcinoma in mice.

Applications

Unspecified application

Species

Mouse

Supriya Sen,Magda Langiewicz,Hassan Jumaa,Nicholas J G Webster

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SRSF3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (AB125124)

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB125124)

Western blot - Anti-SRSF3 antibody (AB125124)

Western blot - Anti-SRSF3 antibody (AB125124)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise