AB73891

Anti-SRSF3 antibody

Name: Anti-SRSF3 antibody (ab73891) | Abcam
Brand: Abcam Limited
SKU: AB73891
Rating: 5 (5 reviews)

(5 Reviews)

(11 Publications)

Rabbit Polyclonal SRSF3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SRSF3.

View Alternative Names

SFRS3, SRP20, SRSF3, Serine/arginine-rich splicing factor 3, Pre-mRNA-splicing factor SRP20

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB73891)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB73891)

Left panel : immunofluorescence analysis of MCF7 cells, using 1/500-1/1000 ab73891; Right panel : same, after pre-incubation with the immunizing peptide.

Unknown

Western blot - Anti-SRSF3 antibody (AB73891)

All lanes:

Western blot - Anti-SRSF3 antibody (ab73891) at 1/500 dilution

Lane 1:

extracts from HeLa cells at 30 µg

Lane 2:

extracts from Jurkat cells at 30 µg

Lane 3:

extracts from Jurkat cells at 30 µg with immunizing peptide

Predicted band size: 19 kDa

Observed band size: 19 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human SRSF3. The exact immunogen used to generate this antibody is proprietary information.

P84103

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed : 17036044, PubMed : 26876937, PubMed : 32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed : 26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed : 11336712, PubMed : 18364396, PubMed : 28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed : 11336712, PubMed : 18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1 : interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed : 28984244).

See full target information SRSF3

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39630148

2024

PDGFRα signaling regulates Srsf3 transcript binding to affect PI3K signaling and endosomal trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas E Forman,Marcin P Sajek,Eric D Larson,Neelanjan Mukherjee,Katherine A Fantauzzo

Journal of biochemical and molecular toxicology 38:e23646 PubMed38345168

2024

SCARB1-encoded circ _0029343 induces p73 splicing to promote growth and metastasis of hepatocellular carcinoma via miR-486-5p/SRSF3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Zhu,Shengya Cao,Jinhui Che,Le Zhao,Zhan Su,Deqiang Li,Ruifeng Pei,Lu Xu,Yiren Ding,Wuyuan Zhou

Development (Cambridge, England) 148: PubMed34184034

2021

Srsf3 mediates alternative RNA splicing downstream of PDGFRα signaling in the facial mesenchyme.

Applications

Unspecified application

Species

Unspecified reactive species

Brenna J C Dennison,Eric D Larson,Rui Fu,Julia Mo,Katherine A Fantauzzo

Brain : a journal of neurology 143:3273-3293 PubMed33141183

2020

Splicing machinery dysregulation drives glioblastoma development/aggressiveness: oncogenic role of SRSF3.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio C Fuentes-Fayos,Mari C Vázquez-Borrego,Juan M Jiménez-Vacas,Leire Bejarano,Sergio Pedraza-Arévalo,Fernando L-López,Cristóbal Blanco-Acevedo,Rafael Sánchez-Sánchez,Oscar Reyes,Sebastián Ventura,Juan Solivera,Joshua J Breunig,María A Blasco,Manuel D Gahete,Justo P Castaño,Raúl M Luque

Cancer discovery 10:1388-1409 PubMed32444465

2020

Posttranslational Regulation of the Exon Skipping Machinery Controls Aberrant Splicing in Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yalu Zhou,Cuijuan Han,Eric Wang,Adam H Lorch,Valentina Serafin,Byoung-Kyu Cho,Blanca T Gutierrez Diaz,Julien Calvo,Celestia Fang,Alireza Khodadadi-Jamayran,Tommaso Tabaglio,Christian Marier,Anna Kuchmiy,Limin Sun,George Yacu,Szymon K Filip,Qi Jin,Yoh-Hei Takahashi,David R Amici,Emily J Rendleman,Radhika Rawat,Silvia Bresolin,Maddalena Paganin,Cheng Zhang,Hu Li,Irawati Kandela,Yuliya Politanska,Hiam Abdala-Valencia,Marc L Mendillo,Ping Zhu,Bruno Palhais,Pieter Van Vlierberghe,Tom Taghon,Iannis Aifantis,Young Ah Goo,Ernesto Guccione,Adriana Heguy,Aristotelis Tsirigos,Keng Boon Wee,Rama K Mishra,Francoise Pflumio,Benedetta Accordi,Giuseppe Basso,Panagiotis Ntziachristos

The Journal of clinical investigation 129:4477-4491 PubMed31393851

2019

Degradation of splicing factor SRSF3 contributes to progressive liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Deepak Kumar,Manasi Das,Consuelo Sauceda,Lesley G Ellies,Karina Kuo,Purva Parwal,Mehak Kaur,Lily Jih,Gautam K Bandyopadhyay,Douglas Burton,Rohit Loomba,Olivia Osborn,Nicholas Jg Webster

eLife 6: PubMed28984244

2017

YTHDC1 mediates nuclear export of N-methyladenosine methylated mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Ian A Roundtree,Guan-Zheng Luo,Zijie Zhang,Xiao Wang,Tao Zhou,Yiquang Cui,Jiahao Sha,Xingxu Huang,Laura Guerrero,Phil Xie,Emily He,Bin Shen,Chuan He

International journal of oncology 51:169-183 PubMed28560430

2017

Fyn/heterogeneous nuclear ribonucleoprotein E1 signaling regulates pancreatic cancer metastasis by affecting the alternative splicing of integrin β1.

Applications

Species

Unspecified reactive species

Peng Jiang,Zhonghu Li,Feng Tian,Xiaowu Li,Jin Yang

American journal of physiology. Cell physiology 308:C289-96 PubMed25428883

2014

TRA2β controls Mypt1 exon 24 splicing in the developmental maturation of mouse mesenteric artery smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxu Zheng,John J Reho,Brunhilde Wirth,Steven A Fisher

Molecular and cellular biology 34:1747-56 PubMed24591653

2014

Homer1 alternative splicing is regulated by gonadotropin-releasing hormone and modulates gonadotropin gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Wang,Maria D Chikina,Hanna Pincas,Stuart C Sealfon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SRSF3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (AB73891)

Western blot - Anti-SRSF3 antibody (AB73891)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

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