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Rabbit Recombinant Monoclonal SRSF3 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252), expandable thumbnail
  • Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Expected
Tested
Tested
Mouse
Tested
Expected
Expected
Tested
Rat
Tested
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

Target data

Function

Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing (PubMed:17036044, PubMed:26876937, PubMed:32440474). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons (PubMed:26876937). Also functions as an adapter involved in mRNA nuclear export (PubMed:11336712, PubMed:18364396, PubMed:28984244). Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity (PubMed:11336712, PubMed:18364396). Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal SRSF3 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR16976

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab251252 is the carrier-free version of Anti-SRSF3 antibody [EPR16976] ab198291.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252), expandable thumbnail

    Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using Anti-SRSF3 antibody [EPR16976] ab198291, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SRSF3 antibody [EPR16976] (Anti-SRSF3 antibody [EPR16976] ab198291) at 1/5000 dilution

    Lane 1: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Lane 2: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Lane 3: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

    Observed band size: 19 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using Anti-SRSF3 antibody [EPR16976] ab198291, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma cell line) labelling SRSF3 with purified Anti-SRSF3 antibody [EPR16976] ab198291 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252), expandable thumbnail

    Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using Anti-SRSF3 antibody [EPR16976] ab198291, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SRSF3 antibody [EPR16976] (Anti-SRSF3 antibody [EPR16976] ab198291) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Rat brain lysate at 10 µg

    Lane 3: C6 (Rat glial tumor) whole cell lysate at 10 µg

    Lane 4: RAW 264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 5: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

    Lane 6: NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa

    Observed band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using Anti-SRSF3 antibody [EPR16976] ab198291, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling SRSF3 with Anti-SRSF3 antibody [EPR16976] ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using Anti-SRSF3 antibody [EPR16976] ab198291, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SRSF3 with Anti-SRSF3 antibody [EPR16976] ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using Anti-SRSF3 antibody [EPR16976] ab198291, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SRSF3 with Anti-SRSF3 antibody [EPR16976] ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (ab251252)

    This data was developed using Anti-SRSF3 antibody [EPR16976] ab198291, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T Cell Leukemia cells from peripheral blood) labeling SRSF3 with Anti-SRSF3 antibody [EPR16976] ab198291 at 1/440 dilution (red) compared with a Rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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