Anti-SRSF3 antibody [EPR16976] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T Cell Leukemia cells from peripheral blood) labeling SRSF3 with ab198291 at 1/440 dilution (red) compared with a Rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
Negative control : Used PBS instead of primary ab.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma cell line) labelling SRSF3 with purified ab198291 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

• WB

Supplier Data

Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SRSF3 antibody [EPR16976] (<a href='/en-us/products/primary-antibodies/srsf3-antibody-epr16976-ab198291'>ab198291</a>) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Lane 3:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

• WB

Lab

Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The 20 kDa band size was observed under Tris-Glycine electrophoresis conditions (4–20% Tris-Glycine gradient gel; Tris Glycine running buffer, pH 8.3). Note : Observed molecular weights can vary with electrophoresis systems.

All lanes:

Western blot - Anti-SRSF3 antibody [EPR16976] (<a href='/en-us/products/primary-antibodies/srsf3-antibody-epr16976-ab198291'>ab198291</a>) at 1/5000 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The 25 kDa band size was observed under Bis-Tris electrophoresis conditions (4–12% Bis-Tris gradient gel; MES running buffer, pH 6.5). Note : Observed molecular weights can vary with electrophoresis systems.

All lanes:

Western blot - Anti-SRSF3 antibody [EPR16976] (<a href='/en-us/products/primary-antibodies/srsf3-antibody-epr16976-ab198291'>ab198291</a>) at 1/5000 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Observed band size: 25 kDa

false

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-SRSF3 antibody [EPR16976] - BSA and Azide free (AB251252)

This data was developed using ab198291, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SRSF3 antibody [EPR16976] (<a href='/en-us/products/primary-antibodies/srsf3-antibody-epr16976-ab198291'>ab198291</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

C6 (Rat glial tumor) whole cell lysate at 10 µg

Lane 4:

RAW 264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 6:

NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false