Mouse Monoclonal SRY/TDF antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SRY.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRY/TDF antibody [1G4] (AB135239), expandable thumbnail](https://content.abcam.com/products/images/sry-tdf-antibody-1g4-ab135239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133542.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRY/TDF antibody [1G4] (AB135239)")
![Flow Cytometry - Anti-SRY/TDF antibody [1G4] (AB135239), expandable thumbnail](https://content.abcam.com/products/images/sry-tdf-antibody-1g4-ab135239--flow-cytometry-img128499.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-SRY/TDF antibody [1G4] (AB135239)")
![Western blot - Anti-SRY/TDF antibody [1G4] (AB135239), expandable thumbnail](https://content.abcam.com/products/images/sry-tdf-antibody-1g4-ab135239--western-blot-img160117.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SRY/TDF antibody [1G4] (AB135239)")
![Western blot - Anti-SRY/TDF antibody [1G4] (AB135239), expandable thumbnail](https://content.abcam.com/products/images/sry-tdf-antibody-1g4-ab135239--western-blot-img86670.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SRY/TDF antibody [1G4] (AB135239)")
Publications
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- Experimental & molecular medicine 51:1-162019Sex-determining region Y (SRY) attributes to gender differences in RANKL expression and incidence of osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKlemen Kodrič et. al.PubMed 31409771
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SRY. Database link Q05066
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/400.00000 | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcriptional regulator that controls a genetic switch in male development (PubMed:11563911). It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (PubMed:16414182, PubMed:16996051). Involved in different aspects of gene regulation including promoter activation or repression (PubMed:9525897). Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3' (PubMed:11563911, PubMed:1425584, PubMed:15170344, PubMed:8159753, PubMed:8265659). SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending (PubMed:11563911, PubMed:1425584, PubMed:15170344, PubMed:16762365, PubMed:8159753, PubMed:8265659). Also involved in pre-mRNA splicing (PubMed:11818535). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity).
Alternative names
TDF, SRY, Sex-determining region Y protein, Testis-determining factor
Recommended products
Mouse Monoclonal SRY/TDF antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SRY.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SRY. Database link Q05066
- Clone number
- 1G4
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRY/TDF antibody [1G4] (ab135239)
Immunohistochemical analysis of Paraffin-embedded Human cervical cancer tissue labelling SRY/TDF with ab135239 at 1/200 dilution followed by DAB staining.
![Flow Cytometry - Anti-SRY/TDF antibody [1G4] (ab135239), expandable thumbnail](https://content.abcam.com/products/images/sry-tdf-antibody-1g4-ab135239--flow-cytometry-img128499.jpg "Flow Cytometry - Anti-SRY/TDF antibody [1G4] (ab135239)")
Flow Cytometry - Anti-SRY/TDF antibody [1G4] (ab135239)
Flow cytometric analysis of HepG2 cells labelling SRY/TDF with ab135239 at 1/200 dilution (green). Negative control (red).
![Western blot - Anti-SRY/TDF antibody [1G4] (ab135239), expandable thumbnail](https://content.abcam.com/products/images/sry-tdf-antibody-1g4-ab135239--western-blot-img160117.jpg "Western blot - Anti-SRY/TDF antibody [1G4] (ab135239)")
Western blot - Anti-SRY/TDF antibody [1G4] (ab135239)
Expected MW is 49.4 kDa.
All lanes: Western blot - Anti-SRY/TDF antibody [1G4] (ab135239) at 1/500 dilution
All lanes: Human SRY/TDF recombinant protein
Predicted band size: 24 kDa
![Western blot - Anti-SRY/TDF antibody [1G4] (ab135239), expandable thumbnail](https://content.abcam.com/products/images/sry-tdf-antibody-1g4-ab135239--western-blot-img86670.jpg "Western blot - Anti-SRY/TDF antibody [1G4] (ab135239)")
Western blot - Anti-SRY/TDF antibody [1G4] (ab135239)
All lanes: Western blot - Anti-SRY/TDF antibody [1G4] (ab135239) at 1/500 dilution
All lanes: NTERA2 cell lysate
Predicted band size: 12 kDa, 24 kDa, 40 kDa, 51 kDa, 69 kDa, 70 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRY/TDF antibody [1G4] (ab135239), expandable thumbnail](https://content.abcam.com/products/images/sry-tdf-antibody-1g4-ab135239--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8018.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRY/TDF antibody [1G4] (ab135239)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRY/TDF antibody [1G4] (ab135239)
Immunohistochemical analysis of Paraffin-embedded Human ovarian cancer tissue labelling SRY/TDF with ab135239 at 1/200 dilution followed by DAB staining.
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