AB140309

Anti-SRY/TDF antibody [OTI3C8]

Name: Anti-SRY/TDF antibody [OTI3C8] (ab140309) | Abcam
Brand: Abcam Limited
SKU: AB140309

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(2 Publications)

Mouse Monoclonal SRY/TDF antibody. Suitable for WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SRY.

View Alternative Names

TDF, SRY, Sex-determining region Y protein, Testis-determining factor

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SRY/TDF antibody [OTI3C8] (AB140309)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SRY/TDF antibody [OTI3C8] (AB140309)

Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SRY/TDF, labelling SRY/TDF with ab140309 at 1/100 dilution.

Supplier Data

Western blot - Anti-SRY/TDF antibody [OTI3C8] (AB140309)

Cell lysates were separated by SDS-PAGE and immunoblotted with ab140309

All lanes:

Western blot - Anti-SRY/TDF antibody [OTI3C8] (ab140309)

Lane 1:

HEK293T cells transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cells transfected with pCMV6-ENTRY SRY cDNA for 48 hrs and lysed at 5 µg

Predicted band size: 24 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3C8

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SRY.

Q05066

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The clone number has been updated from 3C8 to OTI3C8, both clone numbers name the same clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator that controls a genetic switch in male development (PubMed : 11563911). It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (PubMed : 16414182, PubMed : 16996051). Involved in different aspects of gene regulation including promoter activation or repression (PubMed : 9525897). Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3' (PubMed : 11563911, PubMed : 1425584, PubMed : 15170344, PubMed : 8159753, PubMed : 8265659). SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending (PubMed : 11563911, PubMed : 1425584, PubMed : 15170344, PubMed : 16762365, PubMed : 8159753, PubMed : 8265659). Also involved in pre-mRNA splicing (PubMed : 11818535). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity).

See full target information SRY

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0333343 PubMed41004429

2025

Impacts of resistance training combined with vibration training on the IGF-1/PI3K/AKT/FOXO3 axis and clinical outcomes in patients with sarcopenia: A protocol for a randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyang Zhou,Jinfeng Yang,Na Li,Jinying Li,Jianxin Ran,Yan Zheng,Yifan Long,Fang Cheng,Yuanpeng Liao

Heliyon 10:e25234 PubMed38375306

2024

EPO regulates the differentiation and homing of bone marrow mesenchymal stem cells through Notch1/Jagged pathway to treat pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Kang Li,Chongyang Shen,Nianchi Wen,Yicen Han,Lu Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SRY/TDF antibody [OTI3C8]

Immunocytochemistry/ Immunofluorescence - Anti-SRY/TDF antibody [OTI3C8] (AB140309)

Western blot - Anti-SRY/TDF antibody [OTI3C8] (AB140309)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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